Anticorps Polyclonal de lapin anti-UBC

• Phare
• Validé par KD/KO

UBC Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 10457-1-AP

Synonymes

HMG20, Polyubiquitin C, UBC, ubiquitin C

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Galerie de données de validation

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  IHC staining of mouse liver using 10457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 10457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10457-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10457-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 10457-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 10457-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 10457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 10457-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 10457-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 10457-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 10457-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu hépatique de souris, tissu de cancer du poumon humain, tissu de carcinome urothélial humain, tissu d'estomac de rat, tissu d'estomac de souris, tissu hépatique de rat, tissu pancréatique de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

10457-1-AP cible UBC dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBC Protéine recombinante Ag0711
• Nom complet: ubiquitin C
• Masse moléculaire calculée: 60 kDa
• Numéro d’acquisition GenBank: BC008955
• Symbole du gène: UBC
• Identification du gène (NCBI): 7316
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin (Ub) is a small, highly conserved eukaryotic protein that has an essential role in diverse cellular signaling pathways, including targeting proteins for proteasomal degradation. Cellular Ub is comprised of two distinct pools consisting of free Ub and Ub-substrate conjugates. UbC is a polyubiquitine gene that encodes Ub polyprotein. UbC, as stress-inducible polyubiquitin precursor proteins of around 9-11 monomers, is cleaved into monomeric ubiquitin by cellular deubiquitylating enzymes.

Publications

Species	Application	Title
human	WB	PLoS One; Ubiquitin B in cervical cancer: critical for the maintenance of cancer stem-like cell characters.; Authors - Yuan Tian ; KD Validated
		Chem Biol Interact; Proteomic analysis of apoptosis induction by lariciresinol in human HepG2 cells.; Authors - Zhan-Jun Ma