Anticorps Polyclonal de lapin anti-UBA6

• Phare
• Validé par KD/KO

UBA6 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 13211-1-AP

Synonymes

E1 L2, Monocyte protein 4, MOP 4, MOP4, UBA6, UBE1L2, Ubiquitin activating enzyme 6

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Galerie de données de validation

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  WB analysis of HEK-293 using 13211-1-AP

  WB result of UBA6 antibody (13211-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBA6 transfected HEK-293 cells.

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  WB analysis using 13211-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13211-1-AP (UBA6 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13211-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13211-1-AP (UBA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 13211-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13211-1-AP (UBA6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 13211-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13211-1-AP (UBA6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13211-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13211-1-AP (UBA6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13211-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13211-1-AP (UBA6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 13211-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 13211-1-AP (UBA6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu cérébral humain
• Résultats positifs en IHC: tissu testiculaire de souris, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

13211-1-AP cible UBA6 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBA6 Protéine recombinante Ag4000
• Nom complet: ubiquitin-like modifier activating enzyme 6
• Masse moléculaire calculée: 1052 aa, 118 kDa
• Poids moléculaire observé: 118 kDa
• Numéro d’acquisition GenBank: BC031637
• Symbole du gène: UBA6
• Identification du gène (NCBI): 55236
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UBA6(Ubiquitin-like modifier-activating enzyme 6) is also named as MOP4, UBE1L2 and belongs to the ubiquitin-activating E1 family. It probably acts as a single molecule like UBE1 and not as a heterodimeric E1 complex, as formed by AOS1/Uba2 and APP-BP1/Uba3. UBA6 is even more restricted in distribution because it is only found in vertebrates but not in lower organisms so that it might have a specialized role in tissues of higher organisms, and in particular, in the testis, where it is expressed by far most abundantly.(PMID:17580310). It has 4 isoforms produced by alternative splicing. This antibody recognizes the full-length(118 kda) and the truncated isoform CRA_b (60-70 kDa) of UBA6 (NCBI).

Publications

Species	Application	Title
human,mouse	WB,IHC,IP	Nat Commun; Inhibition of UBA6 by inosine augments tumour immunogenicity and responses; Authors - Lei Zhang