Anticorps Polyclonal de lapin anti-TrkB

TrkB Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 29961-1-AP

Synonymes

GP145 TrkB, NTRK2, Trk B, TRKB, TrkB tyrosine kinase

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Galerie de données de validation

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  WB analysis using 29961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29961-1-AP (NTRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29961-1-AP (TrkB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 29961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29961-1-AP (TrkB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 29961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29961-1-AP (TrkB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 29961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29961-1-AP (NTRK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 29961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29961-1-AP (NTRK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 29961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29961-1-AP (NTRK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 29961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29961-1-AP (NTRK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 29961-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TrkB antibody (29961-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu de cervelet de souris
• Résultats positifs en IHC: tissu de cervelet de souris, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

29961-1-AP cible TrkB dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TrkB Protéine recombinante Ag32196
• Nom complet: neurotrophic tyrosine kinase, receptor, type 2
• Masse moléculaire calculée: 822 aa, 92 kDa
• Poids moléculaire observé: 90-95 kDa, 140-145 kDa
• Numéro d’acquisition GenBank: BC031835
• Symbole du gène: NTRK2
• Identification du gène (NCBI): 4915
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. It is a single-pass type 1 membrane protein consisting of three domains: an extracellular ligand binding domain, a transmembrane domain and an intracellular tyrosine kinase domain. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance through regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity. This antibody raised against 238-430 aa of human TrkB recognizes full length form (140-145 kDa) and truncated form (90-95 kDa) of TrkB (PMID: 23526925; 21039416).