Anticorps Polyclonal de lapin anti-TUSC2

TUSC2 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 11538-1-AP

Synonymes

C3orf11, Fus 1 protein, FUS1, Fusion 1 protein, LGCC, PAP, PDAP2, PDGFA associated protein 2, tumor suppressor candidate 2, TUSC2

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Galerie de données de validation

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  WB analysis of human heart using 11538-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11538-1-AP (TUSC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 11538-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11538-1-AP (TUSC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11538-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11538-1-AP (TUSC2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 11538-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11538-1-AP (TUSC2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 11538-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11538-1-AP (TUSC2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 11538-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11538-1-AP (TUSC2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 11538-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11538-1-AP (TUSC2 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A549 using 11538-1-AP

  Immunofluorescent analysis of A549 cells using 11538-1-AP (TUSC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, tissu pancréatique de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

11538-1-AP cible TUSC2 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TUSC2 Protéine recombinante Ag2118
• Nom complet: tumor suppressor candidate 2
• Masse moléculaire calculée: 12 kDa
• Poids moléculaire observé: 10-12 kDa
• Numéro d’acquisition GenBank: BC023976
• Symbole du gène: TUSC2
• Identification du gène (NCBI): 11334
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FUS1 (or TUSC2) gene is a highly conserved lung cancer candidate gene, which was identified in the 120 kb 3p21.3 critical region contained in nested lung and breast cancer homozygous deletions. Overexpression of FUS1 gene leads to G1 arrest and growth inhibition of lung cancer cells (PMID: 11593436). The encoded Fusion-1 protein was down-regulated, mutated or lost in the majority of inflammatory thoracic malignancies. It has been evidenced that Fusion-1 establishes its immune- and tumour-suppressive activities via regulation of mitochondrial homeostasis (PMID: 22513871). In addition, myristoylation is found to be required for Fusion-1-mediated tumor-suppressing activity and suggest a novel mechanism for the inactivation of tumor suppressors in lung cancer and a role for deficient posttranslational modification in tumor suppressor-gene-mediated carcinogenesis (PMID: 15126327).

Publications

Species	Application	Title
human,mouse	WB,IHC,IF	Cancer Lett; NEDD4 degrades TUSC2 to promote glioblastoma progression.; Authors - Tadas K Rimkus
human	WB,IHC	Oncotarget; TWIST1/miR-584/TUSC2 pathway induces resistance to apoptosis in thyroid cancer cells.; Authors - Francesca Maria Orlandella