Anticorps Polyclonal de lapin anti-Gamma Tubulin

• Phare
• Validé par KD/KO

Gamma Tubulin Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris

Applications

WB, IHC, FC (Intra), IP, ELISA, IF

Conjugaison

Non conjugué

Publications

19

Avis (4)

N° de cat : 15176-1-AP

Synonymes

TUBG1, Tubulin gamma 1 chain, TUBG, GCP-1, Gamma-tubulin complex component 1

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Galerie de données de validation

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  WB analysis of HeLa using 15176-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15176-1-AP (gamma Tubulin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 15176-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 15176-1-AP (gamma Tubulin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15176-1-AP

  IP result of anti-Gamma Tubulin (IP:15176-1-AP, 4ug; Detection:15176-1-AP 1:2000) with HeLa cells lysate 1600ug.

• 

  IHC staining of human prostate cancer using 15176-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15176-1-AP (gamma Tubulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15176-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15176-1-AP (gamma Tubulin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 15176-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15176-1-AP (gamma Tubulin antibody at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 15176-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15176-1-AP (gamma Tubulin antibody at dilution of 1:400 (under 40x lens).

• 

  FC experiment of HeLa using 15176-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Gamma Tubulin (15176-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293 transfectées
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu d'hyperplasie de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 13 publications below

Informations sur le produit

15176-1-AP cible Gamma Tubulin dans les applications de WB, IHC, FC (Intra), IP, ELISA, IF et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Gamma Tubulin Protéine recombinante Ag7070
• Nom complet: tubulin, gamma 1
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC000619
• Symbole du gène: Gamma Tubulin
• Identification du gène (NCBI): 7283
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.

Publications

Species	Application	Title
human	IF	Cancer Cell; Hippo Signaling Suppresses Cell Ploidy and Tumorigenesis through Skp2.; Authors - Shihao Zhang
human	IF,WB	Nat Commun; INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex; Authors - Lauren G. Mascibroda
mouse	IF	PLoS Biol; Noncoding RNA Ginir functions as an oncogene by associating with centrosomal proteins.; Authors - Suchismita Panda
mouse	WB	Cell Death Differ; Haploinsufficiency of GCP4 induces autophagy and leads to photoreceptor degeneration due to defective spindle assembly in retina.; Authors - Zhigang Li
human	WB	Elife; LUZP1, a novel regulator of primary cilia and the actin cytoskeleton, is a contributing factor in Townes-Brocks Syndrome.; Authors - Laura Bozal-Basterra
human	WB,IF	J Nanobiotechnology; GRP75-driven, cell-cycle-dependent macropinocytosis of Tat/pDNA-Ca2+ nanoparticles underlies distinct gene therapy effect in ovarian cancer.; Authors - Linjia Su

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Deng (Verified Customer) (07-22-2022)

The cells were fixed with cold methanol.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 : 500
• Cell Tissue Type: Human RPE1 cells

Gamma Tubulin Antibody Immunofluorescence validation (1 : 500 dilution) in Human RPE1 cells (Cat no:15176-1-AP)

FH

Iru (Verified Customer) (06-17-2021)

It works well with O/N incubation.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fallopian tube epithelial cells

Gamma Tubulin Antibody Western Blot validation (1:1000 dilution) in Fallopian tube epithelial cells (Cat no:15176-1-AP)

FH

Christopher (Verified Customer) (07-02-2019)

Very fast delivery I think it arrived in a matter of days which is quick compared to some other companies.Westerns worked really well, so it must be good, over all really pleased with this purchase

• Applications: Western Blot,

FH

Boyan (Verified Customer) (01-29-2019)

Excellent. Works quite well both for WB and IF.

• Applications: Western Blot, Immunofluorescence,

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C