Anticorps Polyclonal de lapin anti-Alpha Tubulin

Alpha Tubulin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 14555-1-AP

Synonymes

acetylated alpha tubulin, TUBA1A, Tubulin alpha 1A chain, EC:3.6.5.-, Detyrosinated tubulin alpha-1A chain

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• Galerie de données de validation
• Proteintech vs. Other Vendors

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  WB analysis of mouse brain using 14555-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14555-1-AP (alpha Tubulin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 14555-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14555-1-AP (alpha Tubulin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14555-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14555-1-AP (alpha Tubulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 14555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14555-1-AP (Alpha Tubulin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14555-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (14555-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HeLa using 14555-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (14555-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu de cervelet de souris
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

14555-1-AP cible Alpha Tubulin dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Alpha Tubulin Protéine recombinante Ag6038
• Nom complet: tubulin, alpha 1a
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC050637
• Symbole du gène: Alpha Tubulin
• Identification du gène (NCBI): 7846
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	J Neurosci Res; Tau nuclear translocation is a leading step in tau pathology process through P53 stabilization and nucleolar dispersion.; Authors - Shaqayeq Roqanian
human	WB	J Cell Sci; ARF6 protects sister chromatid cohesion to ensure the formation of stable kinetochore-microtubule attachments.; Authors - Mohamed Bourmoum
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Dicer1 promotes Aβ clearance via blocking B2 RNA-mediated repression of apolipoprotein E.; Authors - Yan Wang
human	WB,IF	Exp Cell Res; Identification and characterization of INMAP, a novel interphase nucleus and mitotic apparatus protein that is involved in spindle formation and cell cycle progression.; Authors - Shen Enzhi E
human	WB	Cell Rep; SFTSV Infection Induces BAK/BAX-Dependent Mitochondrial DNA Release to Trigger NLRP3 Inflammasome Activation.; Authors - Shufen Li
human	WB	Nanomedicine; Chemosensitization and mitigation of Adriamycin-induced cardiotoxicity using combinational polymeric micelles for co-delivery of quercetin/resveratrol and resveratrol/curcumin in ovarian cancer.; Authors - Adel Al Fatease