Anticorps Polyclonal de lapin anti-Prealbumin/transthyretin

Prealbumin/transthyretin Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

24

Avis (1)

N° de cat : 11891-1-AP

Synonymes

ATTR, HsT2651, PALB, Prealbumin, Prealbumin/transthyretin, TBPA, transthyretin, TTR

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Galerie de données de validation

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  WB analysis using 11891-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11891-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11891-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11891-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11891-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 11891-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 11891-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 11891-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11891-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11891-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11891-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11891-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 11891-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11891-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 11891-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 11891-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 11891-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 11891-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 11891-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 11891-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Prealbumin/transthyretin antibody (11891-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: plasma humain, cellules HEK-293, tissu cardiaque humain, tissu cérébral humain, tissu hépatique humain, tissu splénique humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below
• IHC: See 7 publications below
• IF: See 4 publications below

Informations sur le produit

11891-1-AP cible Prealbumin/transthyretin dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Prealbumin/transthyretin Protéine recombinante Ag2499
• Nom complet: transthyretin
• Masse moléculaire calculée: 147 aa, 16 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC020791
• Symbole du gène: TTR
• Identification du gène (NCBI): 7276
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transthyretin (TTR) is a plasma transport protein for thyroxine and retinol, through the association with retinol-binding protein. It is a homotetrameric protein synthesized mainly in liver, choroid plexus, retinal pigment epithelium, and pancreas.Within the CNS, TTR is the only known protein synthesized solely by the choroid plexus. Mutant and wildtype TTR give rise to various forms of amyloid deposition (amyloidosis). Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR),hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) and carpal tunnel syndrome type 1 (CTS1). In addition, positive immunostaining for TTR has been reported as a sensitive diagnostic marker of choroid plexus tumors. (PMID: 22103483)

Publications

Species	Application	Title
	IF	Cell; Ex utero monkey embryogenesis from blastocyst to early organogenesis; Authors - Yandong Gong
mouse	IHC	Dev Cell; Spatially dependent dynamic MAPK modulation by the Nde1-Lis1-Brap complex patterns mammalian CNS.; Authors - Alison A Lanctot
human	IF	Dev Cell; Lysosomal dynamics regulate mammalian cortical neurogenesis; Authors - Wenzheng Zou
mouse	WB	Theranostics; Growth hormone receptor disrupts glucose homeostasis via promoting and stabilizing retinol binding protein 4.; Authors - Jinxin Liu
	WB	Diabetes; PMID: 25524914; Authors - Laura Zemany
human	WB	Int J Mol Sci; Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest.; Authors - Marta Zarà

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kenzo (Verified Customer) (11-11-2023)

This antibody worked for mouse heart tissue sections with little background.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Heart