Anticorps Polyclonal de lapin anti-BBS8

BBS8 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 12505-1-AP

Synonymes

BBS8, TPR repeat protein 8, TTC8

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Galerie de données de validation

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  WB analysis of mouse testis using 12505-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12505-1-AP (BBS8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12505-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12505-1-AP (BBS8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 12505-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12505-1-AP (BBS8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12505-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12505-1-AP (BBS8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12505-1-AP

  IP result of anti-BBS8 (IP:12505-1-AP, 3ug; Detection:12505-1-AP 1:400) with HeLa cells lysate 2000ug.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules NIH/3T3, tissu pulmonaire humain
• Résultats positifs en IP: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

12505-1-AP cible BBS8 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BBS8 Protéine recombinante Ag3186
• Nom complet: tetratricopeptide repeat domain 8
• Masse moléculaire calculée: 531 aa, 60 kDa
• Poids moléculaire observé: 50-52 kDa
• Numéro d’acquisition GenBank: BC026351
• Symbole du gène: BBS8
• Identification du gène (NCBI): 123016
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BBS8 (also known as TTC8) is a subunit of BBSome complex which is necessary for ciliary membrane biogenesis. BBS8 localizes specifically to ciliated structures, such as the connecting cilium of the retina and columnar epithelial cells in the lung. Defects in BBS8 cause Bardet-Biedl syndrome type 8 (BBS8), an genetic disorder characterised by retinal degeneration, obesity, polydactyly, cognitive impairment and kidney failure. BBS8 generates two alternatively spliced isoforms. Western blot analysis using this antibody detected a major band around 60 kD in lung tissue.