Anticorps Polyclonal de lapin anti-TTC37

TTC37 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 24594-1-AP

Synonymes

KIAA0372, TPR repeat protein 37, TTC37

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Galerie de données de validation

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  WB analysis using 24594-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24594-1-AP (TTC37 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 24594-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24594-1-AP (TTC37 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24594-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24594-1-AP (TTC37 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 24594-1-AP

  IP result of anti-TTC37 (IP:24594-1-AP, 4ug; Detection:24594-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IHC staining of human tonsillitis using 24594-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24594-1-AP (TTC37 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 24594-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24594-1-AP (TTC37 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 24594-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24594-1-AP (TTC37 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 24594-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24594-1-AP (TTC37 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 24594-1-AP

  Immunofluorescent analysis of HepG2 cells using 24594-1-AP (TTC37 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules MCF-7, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu rénal humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

24594-1-AP cible TTC37 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TTC37 Protéine recombinante Ag18442
• Nom complet: tetratricopeptide repeat domain 37
• Masse moléculaire calculée: 1564 aa, 175 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: BC056893
• Symbole du gène: TTC37
• Identification du gène (NCBI): 9652
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TTC37 (tetratricopeptide repeat protein 37), also named as Thespin, encodes 1,564 amino acid protein that contains twenty tetratricopeptide (TPR) repeats. TPR repeat-containing motifs are found in a variety of proteins and may mediate protein-protein interactions and chaperone activity. Although not expressed in liver, TTC37 is a widely expressed protein with highest levels of expression in vascular tissues, lymph node, pituitary, lung and intestine. TTC37 is a component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C). Defects in TTC37 are associated with Trico-Hepato-Enteric (THE) syndrome.

Publications

Species	Application	Title
human,mouse	WB	J Clin Invest; Cytoplasmic RNA quality control failure engages mTORC1-mediated autoinflammatory disease.; Authors - Kun Yang
human	WB	PLoS Genet; A Comprehensive Genomic Analysis Reveals the Genetic Landscape of Mitochondrial Respiratory Chain Complex Deficiencies.; Authors - Masakazu Kohda