Anticorps Polyclonal de lapin anti-TSR2

TSR2 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 16263-1-AP

Synonymes

DT1P1A10, TSR2, WGG1

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Galerie de données de validation

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  WB analysis of HeLa using 16263-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16263-1-AP (TSR2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16263-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16263-1-AP (TSR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16263-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16263-1-AP (TSR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human epididymis using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human epididymis using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human epididymis using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human epididymis using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 16263-1-AP

  Immunofluorescent analysis of MCF-7 cells, using TSR2 antibody 16263-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, tissu rénal de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu cardiaque humain, tissu d'épididyme humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below

Informations sur le produit

16263-1-AP cible TSR2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TSR2 Protéine recombinante Ag9328
• Nom complet: TSR2, 20S rRNA accumulation, homolog (S. cerevisiae)
• Masse moléculaire calculée: 191 aa, 21 kDa
• Poids moléculaire observé: 24-30 kDa
• Numéro d’acquisition GenBank: BC011825
• Symbole du gène: TSR2
• Identification du gène (NCBI): 90121
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IHC	Acta Histochem; Ultrastructural analysis of human leukemia U-937 cells after apoptosis induction: Localization of proteasomes and perichromatin fibers.; Authors - Ekaterina S Snigirevskaya
human	IHC	Cell Biol Int; Ultrastructural traits of apoptosis; Authors - Ekaterina S Snigirevskaya