Anticorps Polyclonal de lapin anti-TSPAN7

TSPAN7 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 18695-1-AP

Synonymes

A15, CCG B7, CD231, Cell surface glycoprotein A15, DXS1692E, Glima, MRX58, MXS1, TALLA 1, tetraspanin 7, TM4SF2, TM4SF2b, Tspan 7, TSPAN7

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Galerie de données de validation

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  WB analysis of K-562 using 18695-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18695-1-AP (TSPAN7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 18695-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18695-1-AP (TSPAN7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 18695-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18695-1-AP (TSPAN7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 18695-1-AP

  IP result of anti-TSPAN7 (IP:18695-1-AP, 4ug; Detection:18695-1-AP 1:500) with K-562 cells lysate 3600ug.

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  IHC staining of human spleen using 18695-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18695-1-AP (TSPAN7 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 18695-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18695-1-AP (TSPAN7 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules K-562
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

18695-1-AP cible TSPAN7 dans les applications de WB, IP, IF, FC, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tetraspanin 7
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28-35 kDa
• Numéro d’acquisition GenBank: BC018036
• Symbole du gène: TSPAN7
• Identification du gène (NCBI): 7102
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TSPAN7, also named as A15, MXS1, CD231, MRX58, CCG-B7, TM4SF2, TALLA-1, TM4SF2b and DXS1692E, is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. TSPAN7 is a cell surface glycoprotein and may have a role in the control of neurite outgrowth. TSPAN7 may be involved in cell proliferation and cell motility. It is not solely expressed in T cells, but also expressed in acute myelocytic leukemia cells of some patients. The antibody only recognize the protein TSPAN7, can't recognize the protein TSPAN6.

Publications

Species	Application	Title
human,rat	WB,FC	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	WB	Adv Sci (Weinh); Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia; Authors - Tiemei Li
human	IHC,WB	Front Oncol; TSPAN7 Exerts Anti-Tumor Effects in Bladder Cancer Through the PTEN/PI3K/AKT Pathway.; Authors - Xi Yu
human	IF	Front Immunol; Functional characterization of TSPAN7 as a novel indicator for immunotherapy in glioma; Authors - Long Chen
human	WB	Front Genet; The biomarkers related to immune infiltration to predict distant metastasis in breast cancer patients; Authors - Chengsi Ren