Anticorps Polyclonal de lapin anti-TSPAN15

TSPAN15 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (2)

N° de cat : 10460-1-AP

Synonymes

2700063A19Rik, NET 7, NET7, Tetraspan NET 7, tetraspanin 15, TM4SF15, Tspan 15, TSPAN15

---

Galerie de données de validation

• 

  WB analysis of human ovary cancer using 10460-1-AP

  human ovary cancer were subjected to SDS PAGE followed by western blot with 10460-1-AP (TSPAN15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cancer de l'ovaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

10460-1-AP cible TSPAN15 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TSPAN15 Protéine recombinante Ag0710
• Nom complet: tetraspanin 15
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC003157
• Symbole du gène: TSPAN15
• Identification du gène (NCBI): 23555
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TSPAN15 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN15 belongs to the TSPANC8 subfamily, which also include TSPAN5, TSPAN10, TSPAN14, TSPAN17, and TSPAN33. It has been reported that TSPANC8 tetraspanins interact with ADAM10 and have a conserved function in the regulation of ADAM10 trafficking and activity, thereby positively regulating Notch receptor activation (PMID: 23091066; 23035126).

Publications

Species	Application	Title
human	IHC,IF	Liver Int; Tspan15-ADAM10 signalling enhances cancer stem cell-like properties and induces chemoresistance via Notch1 activation in ICC; Authors - Arihito Yoshizumi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Maria (Verified Customer) (03-15-2022)

Some unspecific bands in the upper part of the gel but not so much at the expected molecular weight. I used 1:500 solution loading 30ug of protein, but I think it might be possible to use 1:1000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: SH-SY5Y cells

FH

Boyan (Verified Customer) (10-04-2021)

calculated size at 33 and 23 kd. This antibody recognized a very weak band at 23 kd.

• Applications: Western Blot