Anticorps Polyclonal de lapin anti-TSPAN13

• Phare
• Validé par KD/KO

TSPAN13 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 13570-1-AP

Synonymes

NET 6, NET6, Tetraspan NET 6, tetraspanin 13, TM4SF13, Tspan 13, TSPAN13

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Galerie de données de validation

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  WB analysis of mouse lung using 13570-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13570-1-AP (TSPAN13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 13570-1-AP

  IP result of anti-TSPAN13 (IP:13570-1-AP, 3ug; Detection:13570-1-AP 1:300) with mouse lung tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris
• Résultats positifs en IP: tissu pulmonaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

13570-1-AP cible TSPAN13 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TSPAN13 Protéine recombinante Ag4464
• Nom complet: tetraspanin 13
• Masse moléculaire calculée: 204 aa, 22 kDa
• Poids moléculaire observé: 28-35 kDa
• Numéro d’acquisition GenBank: BC033863
• Symbole du gène: TSPAN13
• Identification du gène (NCBI): 27075
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TSPAN13 (also known as NET6 or TM4SF13) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. Overexpression of TSPAN13 has been reported in prostate cancer, suggesting that TSPAN13 may have an important role in the progression of prostate cancer (PMID: 19148481). TSPAN13 has a calcular molecular weight of 22 kDa. This polyclonal antibody is raised against 100-204aa of human TSPAN13. The experimentally determined molecular mass of 28-35 kDa is larger than the calculated molecular mass, which could be a result of post-translational modifications (PMID: 23648579).

Publications

Species	Application	Title
human	WB	Bosn J Basic Med Sci; Downregulation of TSPAN13 by miR-369-3p inhibits cell proliferation in papillary thyroid cancer (PTC).; Authors - Peng Li ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jerome (Verified Customer) (07-08-2020)

Sign background, unclear if specific.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

TSPAN13 Antibody Western Blot, validation (1:1000 dilution) in HEK293T (Cat no:13570-1-AP)