Anticorps Polyclonal de lapin anti-TSKU

TSKU Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 12370-1-AP

Synonymes

E2 induced gene 4 protein, E2IG4, LRRC54, TSK, TSKU, Tsukushi, tsukushin

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Galerie de données de validation

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  WB analysis of mouse liver using 12370-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12370-1-AP (TSKU Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 12370-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 12370-1-AP (TSKU Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12370-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12370-1-AP (TSKU Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 12370-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12370-1-AP (TSKU Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 12370-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12370-1-AP (TSKU Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12370-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12370-1-AP (TSKU Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12370-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12370-1-AP (TSKU Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12370-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12370-1-AP (TSKU Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HepG2, tissu de côlon de souris, tissu pancréatique de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

12370-1-AP cible TSKU dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TSKU Protéine recombinante Ag3054
• Nom complet: tsukushin
• Masse moléculaire calculée: 353 aa, 38 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC020975
• Symbole du gène: TSKU
• Identification du gène (NCBI): 25987
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TSK, also known as Tsukushi, is an atypical member of the small leucine-rich proteoglycan (SLRP) family of extracellular matrix proteins (PMID: 16884925). The human TSK cDNA encodes a 16 amino acid (aa) signal sequence and a 337 aa secreted protein that contains nine LRR, which has a calculated molecular mass of 38kDa and an apparent molecular mass of 40-50kDa cause of the N-glycosylation. In chick embryos, C-TSK is a unique BMP inhibitor that forms a ternary complex with BMP and chordin and is involved in the regulation of primitive streak and node formation. C-TSK migrates as a band of 47 kDa in SDS-PAGE and N-glycosidase F treatment reduces the size to 41 kDa (PMID: 15363410).