Anticorps Polyclonal de lapin anti-TSHR

• Phare
• Validé par KD/KO

TSHR Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 14450-1-AP

Synonymes

CHNG1, hTSHR I, LGR3, Thyrotropin receptor, TSH R, TSHR

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Galerie de données de validation

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  WB analysis of HeLa using 14450-1-AP

  WB result of TSHR antibody (14450-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-TSHR transfected HeLa cells.

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  WB analysis using 14450-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14450-1-AP (TSHR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 14450-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14450-1-AP (TSHR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 14450-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14450-1-AP (TSHR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer using 14450-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14450-1-AP (TSHR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: TT cells, cellules HeLa, tissu de thymus de souris, tissu ovarien de souris
• Résultats positifs en IHC: tissu de cancer de la thyroïde humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

14450-1-AP cible TSHR dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TSHR Protéine recombinante Ag5957
• Nom complet: thyroid stimulating hormone receptor
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 87kDa, 62-70kDa, 28-30kDa
• Numéro d’acquisition GenBank: BC063613
• Symbole du gène: TSHR
• Identification du gène (NCBI): 7253
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Thyroid stimulating hormone (also known as thyrotropin or TSH) is a glycoprotein hormone produced by thyrotrophs in the anterior pituitary gland. The TSH receptor (TSHR), mainly expressed on the thyroid epithelial cells, is a G protein-coupled receptor (GPCR) of the seven-transmembrane domain family and plays a central role in controlling thyroid cell metabolism. The gene of TSHR maps to chromosome 14q31.1, and encodes a 764-amino acid protein with a predicted unmodified molecular weight of 86.8 kDa. The apparent molecular weight of glycosylated form is 95-120 kDa. Studies reveal that TSHR is composed of two subunits, A and B, which are produced by cleavage of single-chain TSHR on the cell surface and subsequently connected by disulfide bonds. This rabbit polyclonal antibody raised against N-terminal region (1-253 aa) of human TSHR can detect glycosylated subunit A with an experimentally determined molecular weight of 62-70 kDa under reducing condition. (PMID: 20578897, 17158770, 12593718)

Publications

Species	Application	Title
human	WB	Theranostics; Obatoclax and LY3009120 Efficiently Overcome Vemurafenib Resistance in Differentiated Thyroid Cancer.; Authors - Wei-Jun Wei
human	IF	Int J Nanomedicine; A TSHR-Targeting Aptamer in Monocytes Correlating with Clinical Activity in TAO; Authors - Jiamin Cao
mouse	IHC	Oxid Med Cell Longev; TSH Combined with TSHR Aggravates Diabetic Peripheral Neuropathy by Promoting Oxidative Stress and Apoptosis in Schwann Cells.; Authors - Jingwen Fan
zebrafish	WB	Environ Pollut; Acute exposure of zebrafish embryo (Danio rerio) to flutolanil reveals its developmental mechanism of toxicity via disrupting the thyroid system and metabolism.; Authors - Miaomiao Teng
human	IF	Cancers (Basel); Patient-Derived Papillary Thyroid Cancer Organoids for Radioactive Iodine Refractory Screening.; Authors - Luc H J Sondorp
human	WB	Front Endocrinol (Lausanne); Long Non-coding Antisense RNA TNRC6C-AS1 Is Activated in Papillary Thyroid Cancer and Promotes Cancer Progression by Suppressing TNRC6C Expression.; Authors - Dilidaer Muhanhali