Anticorps Polyclonal de lapin anti-TSEN2

• Phare
• Validé par KD/KO

TSEN2 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 13103-2-AP

Synonymes

HsSen2, PCH2B, SEN2, SEN2L, tRNA intron endonuclease Sen2, TSEN2

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Galerie de données de validation

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  WB analysis of mouse colon using 13103-2-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 13103-2-AP (TSEN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 13103-2-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 13103-2-AP (TSEN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13103-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13103-2-AP (TSEN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13103-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13103-2-AP (TSEN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 13103-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13103-2-AP (TSEN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu de côlon de souris, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

13103-2-AP cible TSEN2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TSEN2 Protéine recombinante Ag3742
• Nom complet: tRNA splicing endonuclease 2 homolog (S. cerevisiae)
• Masse moléculaire calculée: 465 aa, 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC019582
• Symbole du gène: TSEN2
• Identification du gène (NCBI): 80746
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TSEN2, also named as SEN2, belongs to the tRNA-intron endonuclease family. TSEN2 is a metal ion independent nuclease that cleave the 5' splice site (PMID: 32476018). TSEN2 has 5 isoforms with the molecular mass of 45-53 kDa. TSEN2 is involved in the formation of a human endonuclease complex whose function is the removal of introns from pre-tRNA (PMID: 16595010).

Publications

Species	Application	Title
human	WB	Cell; CLP1 Founder Mutation Links tRNA Splicing and Maturation to Cerebellar Development and Neurodegeneration.; Authors - Ashleigh E Schaffer
human	WB	Am J Hum Genet; Autosomal-Recessive Mutations in the tRNA Splicing Endonuclease Subunit TSEN15 Cause Pontocerebellar Hypoplasia and Progressive Microcephaly.; Authors - Martin W Breuss
human	WB	Genetics; An unknown essential function of tRNA splicing endonuclease is linked to the integrated stress response and intron debranching; Authors - Jennifer E Hurtig ; KD Validated