Anticorps Polyclonal de lapin anti-Tuberin/TSC2

Tuberin/TSC2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 24601-1-AP

Synonymes

LAM, TSC2, TSC4, Tuberin, tuberous sclerosis 2, Tuberous sclerosis 2 protein

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Galerie de données de validation

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  WB analysis using 24601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24601-1-AP (Tuberin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24601-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24601-1-AP (Tuberin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 24601-1-AP

  IP result of anti-Tuberin/TSC2 (IP:24601-1-AP, 4ug; Detection:24601-1-AP 1:1000) with SH-SY5Y cells lysate 1080 ug.

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  IHC staining of mouse brain using 24601-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24601-1-AP (Tuberin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 24601-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24601-1-AP (Tuberin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 24601-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24601-1-AP (Tuberin antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 24601-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24601-1-AP (Tuberin antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 24601-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Tuberin/TSC2 antibody (24601-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 24601-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Tuberin (24601-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules K-562, cellules PC-3, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules SH-SY5Y,
• Résultats positifs en IHC: tissu cérébral de souris, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,
• Résultats positifs en FC (Intra): cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below

Informations sur le produit

24601-1-AP cible Tuberin/TSC2 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Tuberin/TSC2 Protéine recombinante Ag18133
• Nom complet: tuberous sclerosis 2
• Masse moléculaire calculée: 1807 aa, 201 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: BC150300
• Symbole du gène: TSC2
• Identification du gène (NCBI): 7249
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TSC2, also named as TSC4, FLJ43106 and LAM, acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. In complex with TSC1, TSC2 inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. TSC2 implicated as a tumor suppressor. It is involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. TSC2 stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors. Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors.

Publications

Species	Application	Title
		Immunity; 25-Hydroxycholesterol regulates lysosome AMP kinase activation and metabolic reprogramming to educate immunosuppressive macrophages; Authors - Jun Xiao
human	WB	Research (Wash D C); The FTO Mediated N6-Methyladenosine Modification of DDIT4 Regulation with Tumorigenesis and Metastasis in Prostate Cancer; Authors - Yue Zhao
human	WB	Eur Rev Med Pharmacol Sci; Effect of STAT3 mediated epigenetic regulation in pre-eclampsia: an analysis of trial data.; Authors - X-L Zhang
human	WB	J Oncol; Oleanolic Acid Induces Autophagy and Apoptosis via the AMPK-mTOR Signaling Pathway in Colon Cancer.; Authors - Changxiao Hu
human	WB	NPJ Breast Cancer; Resistance to anti-HER2 therapy associated with the TSC2 nonsynonymous variant c.4349 C > G (p.Pro1450Arg) is reversed by CDK4/6 inhibitor in HER2-positive breast cancer; Authors - Ziyan Yang
human	WB	Elife; A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progression; Authors - Chaojun Yan