Anticorps Polyclonal de lapin anti-TRPS1

• Phare
• Validé par KD/KO

TRPS1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, chIP, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 20003-1-AP

Synonymes

GC79, LGCR, TRPS1, Zinc finger protein GC79

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Galerie de données de validation

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  WB analysis of mouse brain using 20003-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20003-1-AP (TRPS1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 20003-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20003-1-AP (TRPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20003-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20003-1-AP (TRPS1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa cells using 20003-1-AP

  IP result of anti-TRPS1 (20003-1-AP for IP and Detection).

• 

  IHC staining of human prostate cancer using 20003-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 20003-1-AP (TRPS1 Antibody) at dilution of 1:50.

• 

  IHC staining of human prostate cancer using 20003-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 20003-1-AP (TRPS1 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 20003-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20003-1-AP (TRPS1 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 20003-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20003-1-AP (TRPS1 Antibody) at dilution of 1:50.

• 

  IHC staining of mouse brain using 20003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20003-1-AP (TRPS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 20003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20003-1-AP (TRPS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 20003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20003-1-AP (TRPS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 20003-1-AP

  Immunofluorescent analysis of HepG2 cells, using TRPS1 antibody 20003-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu rénal de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu cérébral de souris, tissu hépatique de souris, tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• ChIP: See 3 publications below

Informations sur le produit

20003-1-AP cible TRPS1 dans les applications de WB, IP, IF, IHC, chIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: trichorhinophalangeal syndrome I
• Masse moléculaire calculée: 142 kDa
• Poids moléculaire observé: 135-142 kDa
• Numéro d’acquisition GenBank: NM_014112
• Symbole du gène: TRPS1
• Identification du gène (NCBI): 7227
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRPS1, also named as GC79 and LGCR, is a transcriptional repressor. TRPS1 may act to restrict expression of GATA-regulated genes at selected sites and stages in vertebrate development. It might be involved in prostate cancer apoptosis. Defects in TRPS1 are the cause of tricho-rhino-phalangeal syndrome type 1/2/3 (TRPS1) (TRPS2) (TRPS3). The antibody recognizes the C-term of TRPS1.

Publications

Species	Application	Title
mouse	IF	Cell Stem Cell; Multi-omic analysis reveals divergent molecular events in scarring and regenerative wound healing.; Authors - Shamik Mascharak
human	WB	J Pathol; Down-regulation of TRPS1 stimulates epithelial-mesenchymal transition and metastasis through repression of FOXA1.; Authors - Jin-Zhou Huang ; KD Validated
human	IHC	Int J Mol Med; Pro‑differentiating compounds for human intervertebral disc cells are present in Violina pumpkin leaf extracts; Authors - Elisabetta Lambertini
	WB	J Biol Chem; Dual Role of the Trps1 Transcription Factor in Dentin Mineralization.; Authors - Maria Kuzynski ; KD Validated
human	IHC,ChIP	J Cell Physiol; Expression and function of the P2X7 receptor in human osteoblasts: The role of NFATc1 transcription factor.; Authors - Leticia Scussel Bergamin
human	IHC,IF	Life Sci; Decellularized extracellular matrix-based scaffold and hypoxic priming: A promising combination to improve the phenotype of degenerate intervertebral disc cells.; Authors - Letizia Penolazzi