Anticorps Polyclonal de lapin anti-TRPM8

TRPM8 Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 12813-1-AP

Synonymes

LTrpC 6, LTRPC6, Trp p8, TRPM8, TRPP8

---

Galerie de données de validation

• 

  IHC staining of human liver cancer using 12813-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12813-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12813-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12813-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12813-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12813-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12813-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12813-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 12813-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 12813-1-AP (TRPM8 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

---

Applications testées

• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer de la prostate humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

12813-1-AP cible TRPM8 dans les applications de IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRPM8 Protéine recombinante Ag3483
• Nom complet: transient receptor potential cation channel, subfamily M, member 8
• Masse moléculaire calculée: 1104 aa, 128 kDa
• Numéro d’acquisition GenBank: BC001135
• Symbole du gène: TRPM8
• Identification du gène (NCBI): 79054
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRPM8, also known as CMR1 (cold-menthol receptor-1), is a nonselective cation channel activated by cold and the cooling substances menthol and icilin (PMID: 11893340; 11882888). It serves as a neuronal sensor of cold temperatures. Functioning TRPM8 channels are required for behavioral responses to innocuous cool, noxious cold, injury-evoked cold hypersensitivity, and cooling-mediated analgesia (PMID: 17538622; 21438154; 16920620; 21984952). Additionally, TRPM8 has also been reported to be involved in thermoregulation and the regulation of basal tear flow (PMID: 21407809; 21076394). TRPM8 is expressed extensively in sensory nerves, human prostate and overexpressed in a variety of cancers including prostate, breast, lung, colon and skin melanomas (PMID: 11325849; 20816009).

Publications

Species	Application	Title
mouse	IF	Biomaterials; Role of molybdenum in material immunomodulation and periodontal wound healing: Targeting immunometabolism and mitochondrial function for macrophage modulation.; Authors - Xiao-Tao He
mouse	IF	Inflammation; Cold Stimuli Facilitate Inflammatory Responses Through Transient Receptor Potential Melastatin 8 (TRPM8) in Primary Airway Epithelial Cells of Asthmatic Mice.; Authors - Haipei Liu
mouse	IHC,IF	J Dermatol Sci; The temperature-sensitive receptors TRPV4 and TRPM8 have important roles in the pruritus of rosacea; Authors - Xiyuan Zhou
human,mouse	IHC	Cancer Gene Ther; TRPM8 promotes hepatocellular carcinoma progression by inducing SNORA55 mediated nuclear-mitochondrial communication; Authors - Jie Fu