Anticorps Polyclonal de lapin anti-TROVE2

TROVE2 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 12718-1-AP

Synonymes

60 kDa ribonucleoprotein Ro, 60 kDa Ro protein, Ro 60 kDa autoantigen, RO60, RoRNP, Sjoegren syndrome antigen A2, SS A, SSA2, TROVE domain family member 2, TROVE domain family, member 2, TROVE2

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Galerie de données de validation

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  WB analysis of MCF-7 using 12718-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12718-1-AP (TROVE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12718-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12718-1-AP (TROVE2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 12718-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12718-1-AP (TROVE2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 12718-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 12718-1-AP (TROVE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 12718-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12718-1-AP (TROVE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 12718-1-AP

  Immunofluorescent analysis of PC-3 cells using 12718-1-AP (TROVE2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF7, cellules HeLa, cellules PC-3, tissu de thymus de souris, tissu splénique de rat
• Résultats positifs en IF/ICC: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

12718-1-AP cible TROVE2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TROVE2 Protéine recombinante Ag3412
• Nom complet: TROVE domain family, member 2
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC036658
• Symbole du gène: TROVE2
• Identification du gène (NCBI): 6738
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TROVE2, also named as RO60 or RoRNP, is a 538 amino acid protein, which is localized in cytoplasmic mRNP granules containing untranslated mRNAs. TROVE2 as a RNA-binding protein that binds to misfolded non-coding RNAs, pre-5S rRNA, and several small cytoplasmic RNA molecules known as Y RNAs. TROVE2 may stabilize some of these RNAs and protect them from degradation (PubMed:18056422). TROVE2 binds to endogenous Alu retroelements which are induced by type I IFN and stimulate porinflammaotry cytokine secretion.

Publications

Species	Application	Title
human	WB	J Exp Clin Cancer Res; Selective sorting and secretion of hY4 RNA fragments into extracellular vesicles mediated by methylated YBX1 to promote lung cancer progression.; Authors - Chuang Li