Anticorps Polyclonal de lapin anti-TROAP

• Phare
• Validé par KD/KO

TROAP Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 13634-1-AP

Synonymes

TASTIN, TROAP, Trophinin assisting protein, Trophinin associated protein

---

Galerie de données de validation

• 

  WB analysis using 13634-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13634-1-AP (TROAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13634-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13634-1-AP (TROAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 13634-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13634-1-AP (TROAP antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 13634-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13634-1-AP (TROAP antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13634-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13634-1-AP (TROAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13634-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13634-1-AP (TROAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HCT 116, cellules HT-29, cellules Jurkat, cellules PC-3
• Résultats positifs en IHC: tissu de gliome humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IHC: See 5 publications below

Informations sur le produit

13634-1-AP cible TROAP dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TROAP Protéine recombinante Ag4562
• Nom complet: trophinin associated protein (tastin)
• Masse moléculaire calculée: 778 aa, 84 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC032583
• Symbole du gène: TROAP
• Identification du gène (NCBI): 10024
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TROAP, also known as tastin, was first cloned from human epithelial cells in 1995. It as a cytoplasmic protein is composed of 778 amino acid residues and contains potential phosphorylation sites for protein kinases. TROAP highly expresses in bone marrow, testis and thymus, and is involved in centrosome integrity and spindle assembly during mitosis (PMID: 33500384). TROAP has been shown to promote some tumor progressions, such as those of breast and colorectal cancers (PMID: 33692939).

Publications

Species	Application	Title
		Dev Cell; The HCMV Assembly Compartment Is a Dynamic Golgi-Derived MTOC that Controls Nuclear Rotation and Virus Spread.; Authors - Dean J Procter
human	IHC	Brief Bioinform; Comprehensive assessment of cellular senescence in the tumor microenvironment.; Authors - Xiaoman Wang
human	WB	Biomed Pharmacother; MicroRNA-519d-3p inhibits cell proliferation and migration by targeting TROAP in colorectal cancer.; Authors - Xiaoyong Ye ; KD Validated
human	WB	Cell Cycle; MiR-532-3p suppresses cell viability, migration and invasion of clear cell renal cell carcinoma through targeting TROAP.; Authors - Bin Gao
human	WB	Front Oncol; Downregulation of Methyltransferase-Like 14 Promotes Ovarian Cancer Cell Proliferation Through Stabilizing TROAP mRNA.; Authors - Yize Li
human	WB	CNS Neurosci Ther; TROAP regulates cell cycle and promotes tumor progression through Wnt/β-Catenin signaling pathway in glioma cells.; Authors - Zong-Qing Zhao