Anticorps Polyclonal de lapin anti-TRIP13

• Phare
• Validé par KD/KO

TRIP13 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

17

Avis (4)

N° de cat : 19602-1-AP

Synonymes

16E1 BP, 16E1BP, PCH2, TR interacting protein 13, TRIP 13, TRIP13

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Galerie de données de validation

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  WB analysis using 19602-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19602-1-AP (TRIP13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 19602-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19602-1-AP (TRIP13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 19602-1-AP

  IP result of anti-TRIP13 (IP:19602-1-AP, 3ug; Detection:19602-1-AP 1:400) with mouse testis tissue lysate 6000ug.

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Applications testées

• Résultats positifs en WB: cellules HT-1080, cellules Jurkat, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 14 publications below
• IHC: See 5 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

19602-1-AP cible TRIP13 dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIP13 Protéine recombinante Ag6595
• Nom complet: thyroid hormone receptor interactor 13
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC000404
• Symbole du gène: TRIP13
• Identification du gène (NCBI): 9319
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIP13 gene encodes thyroid receptor-interacting protein 13, that interact with thyroid hormone receptors, also known as human papillomavirus type 16 E1 protein-binding protein (16E1BP). This protein belongs to the AAA ATPase family and the PCH2 subfamily. TRIP13 gene may be one of the several playing roles in early-stage non-small cell lung cancer. TRIP13 also functions in chromosome recombination and chromosome structure development during meiosis, and it is required for development of higher-order chromosome structures and is needed for synaptonemal-complex formation.

Publications

Species	Application	Title
mouse	WB	Sci Adv; SKP1 drives the prophase I to metaphase I transition during male meiosis.; Authors - Yongjuan Guan
human	WB	Nat Commun; TRIP13 promotes error-prone nonhomologous end joining and induces chemoresistance in head and neck cancer.; Authors - Rajat Banerjee ; KD Validated
	IF	Cell Prolif; ZFP541 and KCTD19 regulate chromatin organization and transcription programs for male meiotic progression; Authors - Xu Zhou
human	IHC	Mol Oncol; TRIP13 promotes metastasis of colorectal cancer regardless of p53 and microsatellite instability status.; Authors - Sumit Agarwal
human	WB,IHC,IP	Cell Death Dis; TRIP13 promotes tumor growth and is associated with poor prognosis in colorectal cancer.; Authors - Nengquan Sheng
mouse	IF	PLoS Genet; The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility.; Authors - Laura Gómez-H

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iru (Verified Customer) (06-25-2020)

This antibody works pretty well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fallopian tube epithelial cells

FH

Min (Verified Customer) (11-11-2019)

This is very clear and strong antibody

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Head and neck cancer cells

TRIP13 Antibody Western Blot,Immunoprecipitation, validation (1:5000 dilution) in Head and neck cancer cells (Cat no:19602-1-AP)

FH

Iru (Verified Customer) (06-21-2019)

This antibody doesn't work well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Fallopian tube epithelial cells

FH

Priyanka (Verified Customer) (12-18-2018)

• Applications: Western Blot,