Anticorps Polyclonal de lapin anti-TRIP10

• Phare
• Validé par KD/KO

TRIP10 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 10798-1-AP

Synonymes

Cdc42 interacting protein 4, CIP4, HSTP, Protein Felic, Salt tolerant protein, STOT, STP, TR interacting protein 10, TRIP 10, TRIP10

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Galerie de données de validation

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  WB analysis using 10798-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10798-1-AP (TRIP10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10798-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10798-1-AP (TRIP10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 10798-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10798-1-AP (TRIP10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 10798-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10798-1-AP (TRIP10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 10798-1-AP

  IP result of anti-TRIP10 (IP:10798-1-AP, 4ug; Detection:10798-1-AP 1:10000) with K-562 cells lysate 1760 ug.

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  IF Staining of BxPC-3 using 10798-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 10798-1-AP (TRIP10 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10798-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRIP10 antibody (10798-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10798-1-AP

  Immunofluorescent analysis of HeLa cells using 10798-1-AP (TRIP10 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules BxPC-3, cellules C6, cellules HeLa, cellules K-562, cellules NIH/3T3, tissu de muscle squelettique de souris
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IF/ICC: cellules HeLa, cellules BxPC-3

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

10798-1-AP cible TRIP10 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIP10 Protéine recombinante Ag1251
• Nom complet: thyroid hormone receptor interactor 10
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 75-85 kDa
• Numéro d’acquisition GenBank: BC013002
• Symbole du gène: TRIP10
• Identification du gène (NCBI): 9322
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIP10 (thyroid hormone receptor interactor 10), also known as CIP4 (Cdc42-interacting protein 4), is a novel adaptor protein which may be invloved in plasma membrane remodeling. CIP4 has been reported to negatively regulate glucose uptake by promoting GLUT4 endocytosis in muscle cells. At least five splice variants for human CIP4 have been identified, including CIP4a (CIP4), CIP4b (FELIC), CIP4c, CIPh (CIP4/2). Western blot analysis usually demonstrates a major band around 75 kDa and a weaker band about 85 kDa.

Publications

Species	Application	Title
human	WB,IF	Nat Commun; Mechanical loading of intraluminal pressure mediates wound angiogenesis by regulating the TOCA family of F-BAR proteins.; Authors - Shinya Yuge
human	WB,IHC	J Exp Clin Cancer Res; CDC42-interacting protein 4 promotes metastasis of nasopharyngeal carcinoma by mediating invadopodia formation and activating EGFR signaling.; Authors - Dong-Fang Meng ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

anwesh (Verified Customer) (04-01-2019)

Great antibody. Detects human version of the protein in several cell types. 293T, U2OS and A549 cells tested. Antibody also IP's the endogenous protein

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

TRIP10 Antibody Western Blot,Immunoprecipitation, validation (1:1000 dilution) in 293T (Cat no:10798-1-AP)