Anticorps Polyclonal de lapin anti-TRIM46

• Phare
• Validé par KD/KO

TRIM46 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 21026-1-AP

Synonymes

Gene Y protein, GENEY, TRIFIC, TRIM46, tripartite motif containing 46

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Galerie de données de validation

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  WB analysis using 21026-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21026-1-AP (TRIM46 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 21026-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TRIM46 antibody (21026-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

21026-1-AP cible TRIM46 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM46 Protéine recombinante Ag15290
• Nom complet: tripartite motif-containing 46
• Masse moléculaire calculée: 759 aa, 83 kDa
• Poids moléculaire observé: 83 kDa
• Numéro d’acquisition GenBank: BC069416
• Symbole du gène: TRIM46
• Identification du gène (NCBI): 80128
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tripartite motif-containing protein 46 (TRIM46), a microtubule-associated protein, is involved in the formation of parallel microtubule bundles linked by cross-bridges in the proximal axon. Required for the uniform orientation and maintenance of the parallel microtubule fascicles, which are important for efficient cargo delivery and trafficking in axons. Thereby also required for proper axon specification, the establishment of neuronal polarity, and proper neuronal migration. The TRIM46 protein reported in previous studies includes the long isoform TRIM46L (83 kDa) and the short isoform TRIM46S (60 kDa).

Publications

Species	Application	Title
mouse	WB,IP	Nat Commun; Multilayered regulations of alternative splicing, NMD, and protein stability control temporal induction and tissue-specific expression of TRIM46 during axon formation.; Authors - John K Vuong
mouse	WB,IF	Cell Rep; The Spatiotemporal Construction of the Axon Initial Segment via KIF3/KAP3/TRIM46 Transport under MARK2 Signaling.; Authors - Sotaro Ichinose
human	IHC	Oncol Res; Tripartite Motif-Containing 46 Promotes Viability and Inhibits Apoptosis of Osteosarcoma Cells by Activating NF-B Signaling Through Ubiquitination of PPAR.; Authors - Wenwei Jiang
human	WB	Exp Cell Res; TRIM46 contributes to high glucose-induced ferroptosis and cell growth inhibition in human retinal capillary endothelial cells by facilitating GPX4 ubiquitination.; Authors - Jingfa Zhang ; KD Validated
human	WB,IF	Int J Mol Sci; Mmu-miR-1894-3p Inhibits Cell Proliferation and Migration of Breast Cancer Cells by Targeting Trim46.; Authors - Li Zhang
human	WB,CoIP	Eye Vis (Lond); TRIM46 aggravated high glucose-induced hyper permeability and inflammatory response in human retinal capillary endothelial cells by promoting IκBα ubiquitination; Authors - Hangqi Shen ; KO Validated