Anticorps Polyclonal de lapin anti-TRIM38

• Phare
• Validé par KD/KO

TRIM38 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 13405-1-AP

Synonymes

RING finger protein 15, RNF15, RORET, TRIM38, tripartite motif containing 38, Zinc finger protein RoRet

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Galerie de données de validation

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  WB analysis of human liver using 13405-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13405-1-AP (TRIM38 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 13405-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 13405-1-AP (TRIM38 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13405-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13405-1-AP (TRIM38 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 13405-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13405-1-AP (TRIM38 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 13405-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13405-1-AP (TRIM38 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13405-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13405-1-AP (TRIM38 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13405-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13405-1-AP (TRIM38 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of L02 using 13405-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed L02 cells using 13405-1-AP (TRIM38 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu hépatique humain, cellules HEK-293, tissu de côlon humain
• Résultats positifs en IHC: tissu hépatique humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules L02

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

13405-1-AP cible TRIM38 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM38 Protéine recombinante Ag3840
• Nom complet: tripartite motif-containing 38
• Masse moléculaire calculée: 465 aa, 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC026930
• Symbole du gène: TRIM38
• Identification du gène (NCBI): 10475
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The proinflammatory cytokines TNFα and IL-1β play central roles in many diseases, including infectious diseases, autoimmunity, and cancers. TRIM38 belongs to the TRIM protein family that is involved in various biological processes, including cell proliferation and apoptosis, innate immunity, and inflammatory response. It has been shown that TRIM38 acts as a suppressor in TOLL-like receptor (TLR)-mediated IFN-β induction by promoting degradation of TRAF6 and NAP1 through the ubiquitinproteasome system. (PMID:24434549)

Publications

Species	Application	Title
human	IHC	Aging (Albany NY); Tertiary lymphoid structure stratifies glioma into three distinct tumor subtypes.; Authors - Xingwang Zhou
mouse	WB	Int Immunopharmacol; TRIM38 protects chondrocytes from IL-1β-induced apoptosis and degeneration via negatively modulating nuclear factor (NF)-κB signaling.; Authors - Shouye Hu ; KD Validated
mouse	WB,IHC	J Lipid Res; Tripartite motif 38 alleviates the pathological process of NAFLD/NASH by promoting TAB2 degradation; Authors - Xinxin Yao
mouse	WB,CoIP	Phytomedicine; Active fraction of Polyrhachis vicina (Rogers) inhibits osteoclastogenesis by targeting Trim38 mediated proteasomal degradation of TRAF6; Authors - Xiaoliang Feng