Anticorps Polyclonal de lapin anti-TRIM37

• Phare
• Validé par KD/KO

TRIM37 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 13037-1-AP

Synonymes

KIAA0898, MUL, Mulibrey nanism protein, POB1, TEF3, TRIM37, tripartite motif containing 37

---

Galerie de données de validation

• 

  WB analysis using 13037-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13037-1-AP (TRIM37 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13037-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13037-1-AP (TRIM37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13037-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13037-1-AP (TRIM37 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13037-1-AP

  IP result of anti-TRIM37 (IP:13037-1-AP, 4ug; Detection:13037-1-AP 1:800) with mouse brain tissue lysate 1800 ug.

• 

  IHC staining of mouse testis using 13037-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13037-1-AP (TRIM37 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13037-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13037-1-AP (TRIM37 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13037-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13037-1-AP (TRIM37 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 13037-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using TRIM37 antibody (13037-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of U-87 MG using 13037-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using TRIM37 antibody (13037-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

---

Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules MCF-7, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu testiculaire de souris, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7, cellules MG U-87

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

13037-1-AP cible TRIM37 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM37 Protéine recombinante Ag3690
• Nom complet: tripartite motif-containing 37
• Masse moléculaire calculée: 964 aa, 108 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC036012
• Symbole du gène: TRIM37
• Identification du gène (NCBI): 4591
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM37 (tripartite motif containing 37) is a member of the tripartite motif (RING, B-Box, and coiled-coil domains) family . Its gene mutations result in mulibrey nanism, which is a rare, autosomal-recessive growth disorder of prenatal onset, with characteristic dysmorphic features, pericardial constriction, and hepatomegaly .TRIM37 is located on chromosome 17q23, encoding a protein with an estimated molecular weight of 130 kDa or 89kDa. (PMID: 28098873, PMID: 29940807, PMID: 14757854)

Publications

Species	Application	Title
human	IHC	Transl Oncol; TRIM37 promotes gallbladder cancer proliferation by activating the Wnt/β-catenin pathway via ubiquitination of Axin1; Authors - Ming Xu
human	WB,IHC,IP	Exp Cell Res; Aerobic glycolysis and tumor progression of hepatocellular carcinoma are mediated by ubiquitin of P53 K48-linked regulated by TRIM37; Authors - Yuzhen Ge ; KD Validated
human	WB,IP	Biochem Biophys Res Commun; Tripartite motif containing protein 37 involves in thrombin stimulated BV-2 microglial cell apoptosis and interleukin 1β release.; Authors - Chong Han ; KD Validated
human	WB	Mol Cancer Res; Downregulation of BASP1 promotes temozolomide resistance in gliomas via epigenetic activation of the FBXO32/NF-κB/MGMT axis; Authors - Xinyi Liao
mouse	WB,IF	Mol Med; TRIM37 exacerbates hepatic ischemia/reperfusion injury by facilitating IKKγ translocation; Authors - Hang Yang ; KD Validated
human,mouse	WB,IF	Cell Discov; The TRIM37 variants in Mulibrey nanism patients paralyze follicular helper T cell differentiation; Authors - Wangpeng Gu