Anticorps Polyclonal de lapin anti-TRIM32

• Phare
• Validé par KD/KO

TRIM32 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 10326-1-AP

Synonymes

tripartite motif containing 32, RING-type E3 ubiquitin transferase TRIM32, HT2A, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM32

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Galerie de données de validation

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  WB analysis of HEK-293 using 10326-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10326-1-AP (TRIM32 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 10326-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10326-1-AP (TRIM32 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10326-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10326-1-AP (TRIM32 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 10326-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10326-1-AP (TRIM32 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 10326-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10326-1-AP (TRIM32 antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of human colon using 10326-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10326-1-AP (TRIM32 antibody) at dilution of 1:25 (under 40x lens).

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  IF Staining of mouse embryo using 10326-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using TRIM32 antibody (10326-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse embryo using 10326-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 10326-1-AP (TRIM32 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse embryo using 10326-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 10326-1-AP (TRIM32 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of hTERT-RPE1 cells using 10326-1-AP

  IF result (cytoplasm stain) of anti-BBS11 (10326-1-AP; 1:50) with hTERT-RPE1 cell by Dr. Moshe Kim.

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  FC experiment of A431 using 10326-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human TRIM32 (10326-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules NIH/3T3, tissu de côlon de souris
• Résultats positifs en IHC: tissu de lymphome humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu embryonnaire de souris,
• Résultats positifs en IF/ICC: cellules hTERT-RPE1,
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

10326-1-AP cible TRIM32 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM32 Protéine recombinante Ag0401
• Nom complet: tripartite motif-containing 32
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 70-72 kDa
• Numéro d’acquisition GenBank: BC003154
• Symbole du gène: TRIM32
• Identification du gène (NCBI): 22954
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM32(Tripartite motif-containing protein 32) is a widely expressed ubiquitin ligase that is localized to the Z-line in skeletal muscle(PMID:19349376). It promotes PIAS4 ubiquitination and degradation, and by controlling PIAS4 stability, regulates UVB-induced keratinocyte apoptosis through induction of NFkappaB(PMID: 16816390). Western blot analysis detected TRIM32 at an apparent molecular mass of 70-72 kDa.

Publications

Species	Application	Title
human	WB	Sci Adv; An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis.; Authors - Molly M Wilson
mouse,human	IHC	J Am Soc Nephrol; Proteome Analysis of Isolated Podocytes Reveals Stress Responses in Glomerular Sclerosis.; Authors - Sybille Koehler
mouse	WB	Hum Mol Genet; Deficiency of the E3 ubiquitin ligase TRIM32 in mice leads to a myopathy with a neurogenic component.; Authors - Kudryashova Elena E ; KO Validated
human,rat	WB,IF,IP	FASEB J; Ubiquitin ligase TRIM32 promotes dendrite arborization by mediating degradation of the epigenetic factor CDYL.; Authors - Lei Liu ; KD Validated
human	IF	J Cell Sci; TRIM32, but not its muscular dystrophy-associated mutant, positively regulates and is targeted to autophagic degradation by p62/SQSTM1.; Authors - Katrine Stange Overå
human	WB	iScience; A nonautophagic role of ATG5 in regulating cell growth by targeting c-Myc for proteasome-mediated degradation.; Authors - Sheng Li