Anticorps Polyclonal de lapin anti-TRIM22

• Phare
• Validé par KD/KO

TRIM22 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 13744-1-AP

Synonymes

GPSTAF50, RING finger protein 94, RNF94, Staf 50, STAF50, TRIM22, tripartite motif containing 22

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Galerie de données de validation

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  IHC staining of human lung cancer using 13744-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13744-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 13744-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 13744-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13744-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13744-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu pancréatique de souris, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

13744-1-AP cible TRIM22 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM22 Protéine recombinante Ag4683
• Nom complet: tripartite motif-containing 22
• Masse moléculaire calculée: 498 aa, 57 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC035582
• Symbole du gène: TRIM22
• Identification du gène (NCBI): 10346
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tripartite motif containing 22 (TRIM22), a member of the TRIM/RBCC family, has been reported to activate the nuclear factor-kappa B (NF-κB) pathway in unstimulated macrophage cell lines (PMID: 23818111). TRIM22 is involved in the host antiviral response, TRIM22 expression is modulated in response to several other viruses and viral antigens.TRIM22 expression is high in monocytes and early granulocytes but decreases in the lymphocyte and late granulocyte populations and is undetectable in erythroid cells (PMID: 32814880).

Publications

Species	Application	Title
human	WB	Nat Commun; Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen.; Authors - Alice Mac Kain
human	WB,IHC	Oncotarget; TRIM22 confers poor prognosis and promotes epithelial-mesenchymal transition through regulation of AKT/GSK3β/β-catenin signaling in non-small cell lung cancer.; Authors - Li Liu ; KD Validated
human	WB,IHC,IF	Front Cell Infect Microbiol; Constitutive TRIM22 Expression in the Respiratory Tract Confers a Pre-Existing Defence Against Influenza A Virus Infection.; Authors - Matthew Charman ; KD Validated
human	IHC	Immunobiology; Shared biomarkers of multi-tissue origin for primary Sjogren's syndrome and their importance in immune microenvironment alterations; Authors - Tiantian Cai
human	WB	Cell Biol Int; TRIM22 knockdown suppresses chronic myeloid leukemia via inhibiting PI3K/Akt/mTOR signaling pathway.; Authors - Liyin Li ; KD Validated
human	WB	Transl Cancer Res; FOXO3/TRIM22 axis abated the antitumor effect of gemcitabine in non-small cell lung cancer via autophagy induction.; Authors - Yang Wang ; KD Validated