Anticorps Polyclonal de lapin anti-TRIB3

• Phare
• Validé par KD/KO

TRIB3 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

35

Avis (3)

N° de cat : 13300-1-AP

Synonymes

C20orf97, NIPK, SINK, SKIP3, TRB 3, TRB3, TRIB3, Tribbles homolog 3

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Galerie de données de validation

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  WB analysis using 13300-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 13300-1-AP (TRIB3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 13300-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 13300-1-AP (TRIB3 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Phalloidine stains the F-actin.

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Applications testées

• Résultats positifs en WB: cellules L02,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 28 publications below
• IHC: See 12 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

13300-1-AP cible TRIB3 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, singe
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIB3 Protéine recombinante Ag4085
• Nom complet: tribbles homolog 3 (Drosophila)
• Masse moléculaire calculée: 358 aa, 40 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC027484
• Symbole du gène: TRIB3
• Identification du gène (NCBI): 57761
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIB3 is a pseudokinase molecule that affects a number of cellular functions. TRIB3 has been reported to be highly activated in the presence of a variety of stressors, including the deprivation of neurotrophic factors, hypoxia, and ER stress.TRIB3 protein is associated with a good prognosis in human breast cancer patients, possibly due to the fact that TRIB3 is involved in hypoxia tolerance.(PMID: 21704407). TRIB3 protein predicted molecular weights is approximately 39~45 kDa.(PMID: 31191318, PMID: 24414038, PMID: 31578236).

Publications

Species	Application	Title
	WB,IP	J Cachexia Sarcopenia Muscle; Sarcopenia is attenuated by TRB3 knockout in aging mice via the alleviation of atrophy and fibrosis of skeletal muscles.; Authors - Guo-Kai Shang
monkey	IHC	Aging Dis; Age-Related Decline in Expression of Molecular Chaperones Induces Endoplasmic Reticulum Stress and Chondrocyte Apoptosis in Articular Cartilage.; Authors - Li Tan
human	WB	Autophagy; Cannabidiol inhibits human glioma by induction of lethal mitophagy through activating TRPV4.; Authors - Tengfei Huang ; KD Validated
human	WB,IF	Clin Cancer Res; PMID: 31235507; Authors - Jian Lin ; KD Validated
	WB	EMBO Rep; Wnt regulates amino acid transporter Slc7a5 and so constrains the integrated stress response in mouse embryos.; Authors - Nadège Poncet
human	WB	Oxid Med Cell Longev; Hsa_circRNA_0008028 Deficiency Ameliorates High Glucose-Induced Proliferation, Calcification, and Autophagy of Vascular Smooth Muscle Cells via miR-182-5p/TRIB3 Axis; Authors - Lili Shi ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chun (Verified Customer) (12-05-2019)

a good antibody

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2,000
• Cell Tissue Type: HEK293 cells

FH

KARTHIKEYAN (Verified Customer) (11-20-2019)

I used this antibody in mouse whole brain lysate and in brain endothelial cells, in both the system they work really well. i would definitely recommend this product.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000 for WB
• Cell Tissue Type: Mouse brain endothelial cells, mouse brain tissue lysate

TRIB3 Antibody Western Blot, validation (1:1000 for WB dilution) in Mouse brain endothelial cells, mouse brain tissue lysate (Cat no:13300-1-AP)

FH

Jose (Verified Customer) (11-02-2017)

Bright nuclear staining.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Skleletal muscle