Anticorps Polyclonal de lapin anti-TRAF5

• Phare
• Validé par KD/KO

TRAF5 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 12868-1-AP

Synonymes

RING finger protein 84, RNF84, TRAF5

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Galerie de données de validation

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  WB analysis of Jurkat using 12868-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12868-1-AP (TRAF5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 12868-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12868-1-AP (TRAF5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12868-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12868-1-AP (TRAF5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12868-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12868-1-AP (TRAF5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules PC-3, tissu pancréatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below

Informations sur le produit

12868-1-AP cible TRAF5 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRAF5 Protéine recombinante Ag3519
• Nom complet: TNF receptor-associated factor 5
• Masse moléculaire calculée: 557 aa, 64 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC029600
• Symbole du gène: TRAF5
• Identification du gène (NCBI): 7188
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cancer Lett; Up-regulation of OLR1 expression by TBC1D3 through activation of TNFα/NF-κB pathway promotes the migration of human breast cancer cells.; Authors - Bei Wang
human	WB	Biochim Biophys Acta Mol Cell Res; IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5; Authors - Junfeng Du
human	WB	J Cell Mol Med; Down-regulated HDAC3 elevates microRNA-495-3p to restrain epithelial-mesenchymal transition and oncogenicity of melanoma cells via reducing TRAF5.; Authors - Yanbo Ma ; KD Validated
mouse	WB,IHC	Eur J Pharmacol; TRAF5 protects against myocardial ischemia reperfusion injury via AKT signaling.; Authors - Weipan Xu ; KO Validated
human	WB	Med Oncol; DLC-1 induces mitochondrial apoptosis and epithelial mesenchymal transition arrest in nasopharyngeal carcinoma by targeting EGFR/Akt/NF-κB pathway.; Authors - Wei Huang
human,human	WB	Transl Cancer Res; Effects of antisense lncRNA PCBP1-AS1 on biological behaviors of vulvar squamous carcinoma cells by regulating TRAF5 and NF-κB expression; Authors - Hongyu Wang