Anticorps Polyclonal de lapin anti-TPTE

TPTE Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 12982-1-AP

Synonymes

Cancer/testis antigen 44, CT44, PTEN2, TPTE, Tumor antigen BJ HCC 5

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Galerie de données de validation

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  WB analysis of HepG2 using 12982-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12982-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12982-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12982-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 12982-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 12982-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 12982-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 12982-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12982-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12982-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 12982-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12982-1-AP (TPTE Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12982-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12982-1-AP (TPTE Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of BxPC-3 using 12982-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 12982-1-AP (TPTE antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules BxPC-3, cellules HEK-293, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IHC: tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules BxPC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

12982-1-AP cible TPTE dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TPTE Protéine recombinante Ag3606
• Nom complet: transmembrane phosphatase with tensin homology
• Masse moléculaire calculée: 62 kDa, 64 kDa
• Poids moléculaire observé: 62 kDa, 64 kDa
• Numéro d’acquisition GenBank: BC028719
• Symbole du gène: TPTE
• Identification du gène (NCBI): 7179
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TPTE(Transmembrane phosphatase with tensin homology) is also named as CT44, BJ-HCC-5 and belongs to the non receptor class of the protein-tyrosine phosphatase family. The gene encodes a predicted polypeptide of 551 amino acids with at least 2 potential transmembrane domains and a tyrosine phosphatase motif. TPTE may be involved in the signal transduction pathways of the endocrine or spermatogenic function of the testis(PMID:10598804). It is putatively involved in linking the cell membrane to cytoskeleton. This protein is very similar in sequence to PTEN through the phosphatase and C2 domains, but lacks a PDZ binding motif and an extensively phosphorylated C-terminal tail(PMID:17240336). It has 4 isoforms produced by alternative splicing.