Anticorps Polyclonal de lapin anti-TPM4

• Phare
• Validé par KD/KO

TPM4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 13741-1-AP

Synonymes

TM30p1, TPM4, tropomyosin 4, Tropomyosin alpha 4 chain

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Galerie de données de validation

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  WB analysis using 13741-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13741-1-AP (TPM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13741-1-AP

  WB result of TPM4 antibody (13741-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPM4 transfected HeLa cells.

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  IHC staining of human endometrial cancer using 13741-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 13741-1-AP (TPM4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human endometrial cancer using 13741-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 13741-1-AP (TPM4 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, cellules HeLa, tissu de muscle squelettique de rat
• Résultats positifs en IHC: tissu de cancer de l'endomètre humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

13741-1-AP cible TPM4 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TPM4 Protéine recombinante Ag4679
• Nom complet: tropomyosin 4
• Masse moléculaire calculée: 248 aa, 29 kDa
• Poids moléculaire observé: 32-35 kDa
• Numéro d’acquisition GenBank: BC037576
• Symbole du gène: TPM4
• Identification du gène (NCBI): 7171
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TPM4, also named as TM30p1, Tropomyosin 4, Tropomyosin alpha 4 chain. This gene is one gene type of tropomyosin isoforms and encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is a major regulatory protein of cytoskeleton and is localized along actin filaments modulating the interaction of actin and myosin (PubMed: 26315888, PubMed: 32638329). In addition, TPM4 has been reported as an oncoprotein in several malignancies (PubMed: 37459535). The calculated molecular weight of TPM4 is usually approximately 28 kDa. It maybe have two isoforms: the short 30-kDa isoform (TPM4.2) and the long 38-kDa isoform (TPM4.1) (PubMed: 28134622).

Publications

Species	Application	Title
mouse	WB	J Proteomics; Network of brain protein level changes in glutaminase deficient fetal mice.; Authors - Bae Narkhyun N
human	WB	Onco Targets Ther; WHSC1 Promotes Cell Proliferation, Migration, and Invasion in Hepatocellular Carcinoma by Activating mTORC1 Signaling.; Authors - Jingjing Dai
human	WB,IHC	Int J Med Sci; The expression and clinical significance of TPM4 in hepatocellular carcinoma.; Authors - Linjing Li
human	WB	mSphere; Ehrlichia chaffeensis TRP75 Interacts with Host Cell Targets Involved in Homeostasis, Cytoskeleton Organization, and Apoptosis Regulation To Promote Infection.; Authors - Tian Luo
human	WB	Osteoporos Int; Differentially expressed proteins identified by TMT proteomics analysis in bone marrow microenvironment of osteoporotic patients.; Authors - Q Zhou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Marco (Verified Customer) (07-01-2024)

good for westernblot, decent for immunofluorescence

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NRVCMs