Anticorps Polyclonal de lapin anti-TPK1

• Phare
• Validé par KD/KO

TPK1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 10942-1-AP

Synonymes

HTPK1, Placental protein 20, PP20, thiamin pyrophosphokinase 1, Thiamine pyrophosphokinase 1, TPK1

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Galerie de données de validation

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  WB analysis using 10942-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 10942-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10942-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human small intestine using 10942-1-AP

  human small intestine tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10942-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10942-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 10942-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10942-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 10942-1-AP

  IP result of anti-TPK1 (IP:10942-1-AP, 4ug; Detection:10942-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human kidney using 10942-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10942-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10942-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10942-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10942-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10942-1-AP (TPK1 Antibody) at dilution of 1:50(under 10x lens).

• 

  IHC staining of human kidney using 10942-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10942-1-AP (TPK1 Antibody) at dilution of 1:50(under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules HEK-293, cellules L02, tissu d'intestin grêle de rat, tissu d'intestin grêle de souris, tissu d'intestin grêle humain, tissu rénal de souris, tissu testiculaire de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below

Informations sur le produit

10942-1-AP cible TPK1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TPK1 Protéine recombinante Ag1404
• Nom complet: thiamin pyrophosphokinase 1
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC014552
• Symbole du gène: TPK1
• Identification du gène (NCBI): 27010
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TPK1(Thiamin pyrophosphokinase 1) is also named as PP20(placental protein 20) and belongs to the thiamine pyrophosphokinase family. It is a cellular enzyme involved in the regulation of thiamine metabolism and catalyzes the conversion of thiamine, a form of vitamin B1, to thiamine pyrophosphate (TDP, or TPP). There is no difference in TPK1 expression in cultured fibroblasts from normal subjects or from patients with thiamine-responsive megaloblastic anemia(PMID:11342111). It can exsit as a dimer(PMID:21552434). Defects in TPK1 are the cause of thiamine metabolism dysfunction syndrome type 5, episodic encephalopathy type (THMD5).

Publications

Species	Application	Title
human	WB	Sci Adv; Dietary thiamine influences l-asparaginase sensitivity in a subset of leukemia cells.; Authors - Rohiverth Guarecuco ; KO Validated
mouse	WB	Proc Natl Acad Sci U S A; OCT1 is a high-capacity thiamine transporter that regulates hepatic steatosis and is a target of metformin.; Authors - Ligong Chen
human	WB	Oncotarget; Identification of vitamin B1 metabolism as a tumor-specific radiosensitizing pathway using a high-throughput colony formation screen.; Authors - Gaganpreet S Tiwana
human	WB	Am J Pathol; Identification of aurora kinase B and Wee1-like protein kinase as downstream targets of (V600E)B-RAF in melanoma.; Authors - Sharma Arati A
rat	WB	Sci Rep; Thiamine metabolism is critical for regulating correlated growth of dendrite arbors and neuronal somata.; Authors - Huimin Liu
human	WB	PLoS One; Metabolic effects of acute thiamine depletion are reversed by rapamycin in breast and leukemia cells.; Authors - Shuqian Liu