Anticorps Polyclonal de lapin anti-Torsin A

Torsin A Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 10296-1-AP

Synonymes

DQ2, Dystonia 1 protein, DYT1, TOR1A, Torsin 1A, Torsin A, Torsin family 1 member A, TorsinA

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Galerie de données de validation

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  WB analysis of mouse brain using 10296-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10296-1-AP (Torsin A antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10296-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10296-1-AP (Torsin A antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10296-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10296-1-AP (Torsin A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10296-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10296-1-AP (Torsin A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10296-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10296-1-AP (Torsin A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 10296-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10296-1-AP (Torsin A antibody) at dilution of 1:100 (under 25x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HEK-293, tissu cérébral de rat, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

10296-1-AP cible Torsin A dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Torsin A Protéine recombinante Ag0358
• Nom complet: torsin family 1, member A (torsin A)
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 42-50 kDa
• Numéro d’acquisition GenBank: BC000674
• Symbole du gène: TOR1A
• Identification du gène (NCBI): 1861
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TOR1A (torsin A), also known as DYT1, is a member of AAA+ family of ATPases that mediate chaperone and other functions involved in conformational modeling of proteins, protection from stress, and targeting of proteins to cellular organelles. TOR1A is a 37 kDa ATP binding protein highly expressed in hippocampus, spinal cord, kidney and liver. TOR1A primarily localizes to the ER while exposure to oxidative stress causes the redistribution to cell surface protrusions. Mutations in gene encoding TOR1A cause the most severe form of hereditary dystonia, termed early onset, generalized dystonia. Interestingly, Lewy bodies of PD patients were intensely immunoreactive for torsinA.

Publications

Species	Application	Title
mouse	WB	Neurobiol Dis; Postnatal Foxp2 regulates early psychiatric-like phenotypes and associated molecular alterations in the R6/1 transgenic mouse model of Huntington's disease; Authors - Ened Rodríguez-Urgellés