Anticorps Polyclonal de lapin anti-TOP1

TOP1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 20705-1-AP

Synonymes

DNA topoisomerase 1, DNA topoisomerase I, TOP1, TOPI, Top-I, TOPO1, topoisomerase (DNA) I

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Galerie de données de validation

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  WB analysis using 20705-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20705-1-AP (TOP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 20705-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOP1 antibody (20705-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules L02,
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer du col de l'utérus humain, tissu de cancer du côlon humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

20705-1-AP cible TOP1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: topoisomerase (DNA) I
• Masse moléculaire calculée: 91 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: NM_003286
• Symbole du gène: TOP1
• Identification du gène (NCBI): 7150
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TOP1 belongs to the eukaryotic type I topoisomerase family. The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. The antibody is specific to TOP1.

Publications

Species	Application	Title
human	WB,IF	Nat Commun; Topoisomerase 1 cleavage complex enables pattern recognition and inflammation during senescence.; Authors - Bo Zhao
human	WB, IF	Cell Chem Biol; Lipid-derived electrophiles mediate the effects of chemotherapeutic topoisomerase I poisons.; Authors - Amy Flor
human	WB	J Transl Med; Integrated multi-omics analyses reveal Jorunnamycin A as a novel suppressor for muscle-invasive bladder cancer by targeting FASN and TOP1; Authors - Ruijiao Chen
human	WB	Oncotarget; Genetic variants in microRNAs predict non-small cell lung cancer prognosis in Chinese female population in a prospective cohort study.; Authors - Xia Lingzi
human	WB	J Cell Mol Med; EGCG synergizes the therapeutic effect of irinotecan through enhanced DNA damage in human colorectal cancer cells.; Authors - Wenbing Wu
mouse	WB	iScience; Sensitization of ovarian tumor to immune checkpoint blockade by boosting senescence-associated secretory phenotype.; Authors - Xue Hao