Anticorps Monoclonal anti-TOM70

TOM70 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

1H8A1

Avis (1)

N° de cat : 66593-1-Ig

Synonymes

KIAA0719, KIAA0719, TOM70, TOM70, TOMM70A

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Galerie de données de validation

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  WB analysis using 66593-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66593-1-Ig (TOM70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  IHC staining of mouse brain using 66593-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66593-1-Ig (TOM70 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66593-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66593-1-Ig (TOM70 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 66593-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66593-1-Ig (TOM70 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 66593-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66593-1-Ig (TOM70 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66593-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66593-1-Ig (TOM70 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules 4T1, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, cellules PC-12
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

66593-1-Ig cible TOM70 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TOM70 Protéine recombinante Ag6027
• Nom complet: translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 67 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC052994
• Symbole du gène: TOMM70A
• Identification du gène (NCBI): 9868
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TOM70 is a mitochondrial import receptor responsible for the import of cytosol synthesized precursor proteins into the mitochondria. Tom70 is a member of the tetratricopeptide repeat (TPR) co-chaperone family and contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90. Recently overexpression of TOM70 has been linked to chemoresistance to drugs in breast cancer cell lines. It is also believed that Tom70 is significantly associated with breast cancer relapse and may predict the recurrence of ovarian cancer.

Publications

Species	Application	Title
human	WB	Science; Structural insight into the SAM-mediated assembly of the mitochondrial TOM core complex.; Authors - Qiang Wang
human	WB	Mol Cell; Dynamic Regulation of Mitochondrial Import by the Ubiquitin System.; Authors - Lilian Phu
mouse	WB	Physiol Rep; TOM complex-independent transport pathway of myoglobin into mitochondria in C2C12 myotubes; Authors - Rikuhide Koma
mouse	IF	Asian J Androl; Caseinolytic mitochondrial matrix peptidase X is essential for homologous chromosome synapsis and recombination during meiosis of male mouse germ cells; Authors - Hai-Wei Feng ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Daniela (Verified Customer) (10-04-2022)

Good specific antibody that shows only one band at 75KDa in HeLa cell lysate (15ug/lane).

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

TOM70 Antibody Western Blot,Immunoprecipitation validation (1:1000 dilution) in HeLa (Cat no:66593-1-Ig)