Anticorps Polyclonal de lapin anti-TNPO2

• Phare
• Validé par KD/KO

TNPO2 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 17831-1-AP

Synonymes

IPO3, Karyopherin beta 2b, KPNB2B, TNPO2, transportin 2, TRN2

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Galerie de données de validation

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  WB analysis of fetal human brain using 17831-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17831-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17831-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 17831-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 17831-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 17831-1-AP

  Immunofluorescent analysis of MCF-7 cells, using TNPO2 antibody 17831-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

17831-1-AP cible TNPO2 dans les applications de WB, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TNPO2 Protéine recombinante Ag12229
• Nom complet: transportin 2
• Masse moléculaire calculée: 887 aa, 100 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: BC072420
• Symbole du gène: TNPO2
• Identification du gène (NCBI): 30000
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IF	Cell Rep; Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function.; Authors - Eva-Maria Hock
human	WB,IF	Sci Rep; Transportin-2 plays a critical role in nucleocytoplasmic shuttling of oestrogen receptor-α.; Authors - Tetsuji Moriyama ; KD Validated
mouse	WB	eLife; Kap-β2/Transportin mediates β-catenin nuclear transport in Wnt signaling; Authors - Woong Y Hwang
human	WB,CoIP	Int J Biol Macromol; Membrane-bound transcription factor LRRC4 inhibits glioblastoma cell motility; Authors - Yang Liu