Anticorps Polyclonal de lapin anti-Transportin-1

Transportin-1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 20679-1-AP

Synonymes

TNPO1, transportin 1, Transportin1, Transportin-1

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Galerie de données de validation

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  WB analysis of HeLa using 20679-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20679-1-AP (Transportin-1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20679-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20679-1-AP (Transportin-1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 20679-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20679-1-AP (Transportin-1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20679-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20679-1-AP (Transportin-1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules L02, tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

20679-1-AP cible Transportin-1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: transportin 1
• Masse moléculaire calculée: 102 kDa
• Poids moléculaire observé: 102 kDa
• Numéro d’acquisition GenBank: NM_002270
• Symbole du gène: Transportin-1
• Identification du gène (NCBI): 3842
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TNPO1, also named as KPNB2, MIP1, TRN and MIP, belongs to the importin beta family. TNPO1 functions in nuclear protein import as nuclear transport receptor. It serves as receptor for nuclear localization signals (NLS) in cargo substrates. TNPO1 is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. TNPO1 is involved in nuclear import of M9-containing proteins. In vitro, it binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. It is involved in hnRNP A1/A2 nuclear export. TNPO1 mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. It binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, it mediates nuclear import of H2A, H2B, H3 and H4 histones, and SRP19. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. The antibody is specific to TNPO1.

Publications

Species	Application	Title
human	WB,IF	PLoS Genet; MxB inhibits long interspersed element type 1 retrotransposition.; Authors - Yu Huang
human	IF	Sci Rep; Nuclear RNA transcript levels modulate nucleocytoplasmic distribution of ALS/FTD-associated protein FUS.; Authors - Yueh-Lin Tsai
human	IF	Cell Rep; Antiviral Immune Response as a Trigger of FUS Proteinopathy in Amyotrophic Lateral Sclerosis.; Authors - Tatyana A Shelkovnikova
human	WB	Small; Effects of Puerarin-Loaded Tetrahedral Framework Nucleic Acids on Osteonecrosis of the Femoral Head; Authors - Yuxuan Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

María (Verified Customer) (02-08-2022)

Works good for WB (1:1000). For IF, some background but works ok (1:100)

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RPE-1/MSC