Anticorps Polyclonal de lapin anti-TIPE2

• Phare
• Validé par KD/KO

TIPE2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

35

N° de cat : 15940-1-AP

Synonymes

TIPE2, TNF alpha-induced protein 8-like protein 2, TNFAIP8 like protein 2, TNFAIP8L2, TNFAIP8-like protein 2, Tumor necrosis factor alpha-induced protein 8-like protein 2, Inflammation factor protein 20

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Galerie de données de validation

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  WB analysis using 15940-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15940-1-AP (TIPE2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 15940-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 15940-1-AP (TIPE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 15940-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 15940-1-AP (TIPE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lymph using 15940-1-AP

  rat lymph tissue were subjected to SDS PAGE followed by western blot with 15940-1-AP (TIPE2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 15940-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 15940-1-AP (TIPE2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 15940-1-AP

  IP result of anti-TIPE2 (IP:15940-1-AP, 4ug; Detection:15940-1-AP 1:2000) with mouse spleen tissue lysate 1400 ug.

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  IHC staining of human lymphoma using 15940-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15940-1-AP (TIPE2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 15940-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15940-1-AP (TIPE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 15940-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15940-1-AP (TIPE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 15940-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15940-1-AP (TIPE2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 15940-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 15940-1-AP (TIPE2 antibody at dilution of 1:50.

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  IHC staining of human ovary tumor using 15940-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 15940-1-AP (TIPE2 antibody at dilution of 1:50.

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  IF Staining of RAW 264.7 using 15940-1-AP

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using TIPE2 antibody (15940-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 15940-1-AP

  Immunofluorescent analysis of HepG2 cells using 15940-1-AP (TIPE2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of THP-1 using 15940-1-AP

  1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human TIPE2 (15940-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules THP-1, cellules RAW 264.7, tissu de cervelet de souris, tissu d'intestin grêle de souris, tissu lymphatique de rat, tissu splénique de rat, tissu splénique de souris
• Résultats positifs en IP: tissu splénique de souris,
• Résultats positifs en IHC: tissu de lymphome humain, tissu de tumeur ovarienne humain, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules RAW 264.7, cellules HepG2
• Résultats positifs en FC (Intra): cellules THP-1
• Résultats positifs en cytométrie: cellules THP-1

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 10 publications below
• WB: See 31 publications below
• IHC: See 12 publications below
• IF: See 10 publications below
• IP: See 2 publications below

Informations sur le produit

15940-1-AP cible TIPE2 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TIPE2 Protéine recombinante Ag7964
• Nom complet: tumor necrosis factor, alpha-induced protein 8-like 2
• Masse moléculaire calculée: 184 aa, 21 kDa
• Poids moléculaire observé: 18-20 kDa
• Numéro d’acquisition GenBank: BC063014
• Symbole du gène: TIPE2
• Identification du gène (NCBI): 79626
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TIPE2 (TNF-α-inducible protein 8-like 2, or TNFAIP8L2), a negative immune regulator, is essential for maintaining immune homeostasis. TIPE2 shares considerable sequence homology with members of the tumor necrosis factor-α-inducible protein 8 (TNFAIP8) family, which are thought to regulate cellular and immune homeostasis. TIPE2 acts as a negative regulator of Toll-like receptor and T-cell receptor function and is preferentially expressed in lymphoid tissues. TIPE2 plays an essential role for a signal transduction pathway that links inflammatory immune response to specific conditions after cerebral ischemia

Publications

Species	Application	Title
human	WB	Nat Immunol; Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.; Authors - Li Jianhua J
mouse	WB,IF,IP	Nat Immunol; Direction of leukocyte polarization and migration by the phosphoinositide-transfer protein TIPE2.; Authors - Svetlana A Fayngerts ; KO Validated
mouse,human	WB	Sci Adv; TIPE2 is a checkpoint of natural killer cell maturation and antitumor immunity.; Authors - Jiacheng Bi ; KO Validated
mouse	WB	Autophagy; TNFAIP8L2/TIPE2 impairs autolysosome reformation via modulating the RAC1-MTORC1 axis.; Authors - Wen Li ; KO Validated
mouse	WB	J Exp Med; TIPE2 specifies the functional polarization of myeloid-derived suppressor cells during tumorigenesis.; Authors - Dehong Yan ; KO Validated
human,mouse	WB	Cancer Immunol Res; Tumor Necrosis Factor-α-Induced Protein 8-Like 2 Fosters Tumor-Associated Microbiota to Promote the Development of Colorectal Cancer.; Authors - Yunwei Lou