Anticorps Polyclonal de lapin anti-TNFAIP8

• Phare
• Validé par KD/KO

TNFAIP8 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 15790-1-AP

Synonymes

GG2 1, MDC 3.13, NDED, SCC S2, SCCS2, TNF alpha induced protein 8, TNF induced protein GG2 1, TNFAIP8

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Galerie de données de validation

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  WB analysis using 15790-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15790-1-AP (TNFAIP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15790-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15790-1-AP (TNFAIP8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-13 using 15790-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 15790-1-AP (TNFAIP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 15790-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 15790-1-AP (TNFAIP8 Antibody) at dilution of 1:50.

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  IHC staining of human tonsillitis using 15790-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 15790-1-AP (TNFAIP8 Antibody) at dilution of 1:50.

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  IHC staining of human lung cancer using 15790-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 15790-1-AP (TNFAIP8 Antibody) at dilution of 1:50.

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  IHC staining of human tonsillitis using 15790-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 15790-1-AP (TNFAIP8 Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules A549, cellules MOLT-4, cellules PC-13
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

15790-1-AP cible TNFAIP8 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TNFAIP8 Protéine recombinante Ag8491
• Nom complet: tumor necrosis factor, alpha-induced protein 8
• Masse moléculaire calculée: 198 aa, 23 kDa
• Poids moléculaire observé: 19-23 kDa
• Numéro d’acquisition GenBank: BC007014
• Symbole du gène: TNFAIP8
• Identification du gène (NCBI): 25816
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TNFAIP8 (tumor necrosis factor, alpha-induced protein 8), also called SCC-S2/GG2-1/NDED, is associated with enhanced cell survival and inhibition of apoptosis. The induction of TNFAIP8 by TNF depends on the activation of NFκB. TNFAIP8 suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation. TNFAIP8 is expressed in adult spleen, lymph node, thymus, thyroid, bone marrow, and placenta, and in fetal liver, lung, and kidney at high levels, also expressed in various tumor tissues and all cancer cell lines tested. There are four major isoforms of TNFAIP8.

Publications

Species	Application	Title
	WB	J Immunol; Crucial Roles of TNFAIP8 Protein in Regulating Apoptosis and Listeria Infection.; Authors - Thomas P Porturas
human	WB	Oncotarget; TNFAIP8 promotes prostate cancer cell survival by inducing autophagy.; Authors - Suryakant Niture ; KD Validated
human	WB	Toxins (Basel); Cyanotoxins Increase Cytotoxicity and Promote Nonalcoholic Fatty Liver Disease Progression by Enhancing Cell Steatosis; Authors - Suryakant Niture
human	IHC	J Proteome Res; Proteomic Analysis of Plasma sEVs Reveals That TNFAIP8 Is a New Biomarker of Cell Proliferation in Diabetic Retinopathy.; Authors - Jing Xiao ; KD Validated
mouse	IF	Sci Rep; The TIPE Molecular Pilot That Directs Lymphocyte Migration in Health and Inflammation.; Authors - Honghong Sun ; KO Validated
mouse	WB	Sci Rep; The Tnfaip8-PE complex is a novel upstream effector in the anti-autophagic action of insulin.; Authors - Ji-Soo Kim ; KD Validated