Anticorps Polyclonal de lapin anti-TMEM175

• Phare
• Validé par KD/KO

TMEM175 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(19)

N° de cat : 19925-1-AP

Synonymes

Transmembrane protein 175, hTMEM175

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Galerie de données de validation

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  WB analysis using 19925-1-AP

  Boiled and unboiled HeLa cell lysates were subjected to SDS PAGE followed by western blot with 19925-1-AP (TMEM175 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 19925-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19925-1-AP (TMEM175 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 19925-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19925-1-AP (TMEM175 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19925-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19925-1-AP (TMEM175 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules SH-SY5Y
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 18 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

19925-1-AP cible TMEM175 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TMEM175 Protéine recombinante Ag13890
• Nom complet: transmembrane protein 175
• Masse moléculaire calculée: 504 aa, 56 kDa
• Poids moléculaire observé: 41-45 kDa, 54-60 kDa
• Numéro d’acquisition GenBank: BC005158
• Symbole du gène: TMEM175
• Identification du gène (NCBI): 84286
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability. It has two isoforms with MW 41-45 kDa and 54-60 kDa. For optimal WB detection with this antibody, we recommend avoiding boiling the sample after lysis.

Publications

Species	Application	Title
	WB	Nature; A growth-factor-activated lysosomal K+ channel regulates Parkinson's pathology.; Authors - Jinhong Wie ; KO Validated
human	WB	Cell; TMEM175 Is an Organelle K(+) Channel Regulating Lysosomal Function.; Authors - Chunlei Cang
human	WB	Ann Neurol; Genetic, Structural, and Functional Evidence Link TMEM175 to Synucleinopathies.; Authors - Lynne Krohn
human	WB	Proc Natl Acad Sci U S A; TMEM175 deficiency impairs lysosomal and mitochondrial function and increases α-synuclein aggregation.; Authors - Sarah Jinn ; KO Validated
mouse	WB	Mol Med; Modulation of anti-cardiac fibrosis immune responses by changing M2 macrophages into M1 macrophages; Authors - Shiqi Chen
		Hum Mol Genet; Mitochondrial clearance and maturation of autophagosomes are compromised in LRRK2 G2019S familial Parkinson's disease patient fibroblasts.; Authors - Joanna A Korecka

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