Anticorps Polyclonal de lapin anti-TINAGL1

• Phare
• Validé par KD/KO

TINAGL1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 12077-1-AP

Synonymes

ARG1, AZ1, GIS5, LCN7, LIECG3, OLRG 2, OLRG2, PSEC0088, TIN Ag related protein, TIN Ag RP, TINAGL, TINAGL1, TINAGRP, UNQ204/PRO230

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Galerie de données de validation

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  WB analysis using 12077-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12077-1-AP (TINAGL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 12077-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12077-1-AP (TINAGL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human adrenal gland using 12077-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 12077-1-AP (TINAGL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 12077-1-AP

  IP result of anti-TINAGL1 (IP:12077-1-AP, 4ug; Detection:12077-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human liver cancer using 12077-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12077-1-AP (TINAGL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12077-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12077-1-AP (TINAGL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 12077-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TINAGL1 antibody (12077-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, tissu de glande surrénale humain
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du foie humain,

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:1500
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

12077-1-AP cible TINAGL1 dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TINAGL1 Protéine recombinante Ag2721
• Nom complet: tubulointerstitial nephritis antigen-like 1
• Masse moléculaire calculée: 467 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC009048
• Symbole du gène: TINAGL1
• Identification du gène (NCBI): 64129
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tubulointerstitial nephritis antigen-like 1 (Tinagl1), a secreted extracellular protein, was initially identified as a putative component of the ECM. Tinagl1 is most commonly associated with embryonic development, including the heart and adrenal glands. Low levels of Tinagl1 expression can be dire for craniofacial development and for female fertility.

Publications

Species	Application	Title
human,mouse	WB,IHC	FASEB J; Down-regulated TINAGL1 in fibroblasts impairs wound healing in diabetes.; Authors - Wen-Qing Tian
human,mouse	WB	Biochim Biophys Acta Mol Cell Res; Functional analysis reveals that Tinagl1 is required for normal muscle development in mice through the activation of ERK signaling.; Authors - Yoriko Sato ; KD Validated
human	WB,IP	J Transl Med; Tubulointerstitial nephritis antigen-like 1 from cancer-associated fibroblasts contribute to the progression of diffuse-type gastric cancers through the interaction with integrin β1; Authors - Dagyeong Lee
human	WB	Cancer Cell; Tinagl1 Suppresses Triple-Negative Breast Cancer Progression and Metastasis by Simultaneously Inhibiting Integrin/FAK and EGFR Signaling.; Authors - Minhong Shen
human	WB	Cancer Manag Res; TINAGL1 promotes hepatocellular carcinogenesis through the activation of TGF-β signaling-medicated VEGF expression.; Authors - Lu Sun ; KD Validated