Anticorps Polyclonal de lapin anti-TIMP-2

TIMP-2 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

37

N° de cat : 17353-1-AP

Synonymes

CSC 21K, Metalloproteinase inhibitor 2, TIMP 2, TIMP2, TIMP-2

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Galerie de données de validation

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  IHC staining of human kidney using 17353-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17353-1-AP (TIMP-2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 17353-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17353-1-AP (TIMP-2 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse lung using 17353-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 17353-1-AP (TIMP-2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 17353-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 17353-1-AP (TIMP-2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 17353-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17353-1-AP (TIMP-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu rénal humain, tissu de cancer de la prostate humain, tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 8 publications below
• IF: See 1 publications below

Informations sur le produit

17353-1-AP cible TIMP-2 dans les applications de IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TIMP-2 Protéine recombinante Ag10051
• Nom complet: TIMP metallopeptidase inhibitor 2
• Masse moléculaire calculée: 220 aa, 24 kDa
• Numéro d’acquisition GenBank: BC052605
• Symbole du gène: TIMP2
• Identification du gène (NCBI): 7077
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The TIMPs are natural highly specific endogenous inhibitors of MMPs. Human TIMP family consists of four 21-28 kDa proteins known as TIMP1, TIMP2, TIMP3, and TIMP4 encoded by four paralogous genes.TIMPs are vital to the maintenance of ECM homeostasis primarily through their MMP inhibitory functions. TIMP2, a member of the TIMP family, regulates the proteolytic activity of all MMPs and is involved in cell differentiation, growth, migration, angiogenesis, and apoptosis. Urinary TIMP2 has been recently recognized as an early biomarker to predict Acute kidney injury (AKI) in critically ill patients.

Publications

Species	Application	Title
human	IHC,IF	Clin Cancer Res; RAB37 Hypermethylation Regulates Metastasis and Resistance to Docetaxel-Based Induction Chemotherapy in Nasopharyngeal Carcinoma.; Authors - Yingqin Li
human		Cell Death Dis; α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation; Authors - Hao Wang
mouse		Int J Mol Sci; PTEN Inhibitor Treatment Lowers Muscle Plasma Membrane Damage and Enhances Muscle ECM Homeostasis after High-Intensity Eccentric Exercise in Mice; Authors - Baile Wu
human		Int J Biol Sci; TDG suppresses the migration and invasion of human colon cancer cells via the DNMT3A/TIMP2 axis.; Authors - Jiyu Miao
human		Mar Drugs; 11-epi-Sinulariolide Acetate Reduces Cell Migration and Invasion of Human Hepatocellular Carcinoma by Reducing the Activation of ERK1/2, p38MAPK and FAK/PI3K/AKT/mTOR Signaling Pathways.; Authors - Jen-Jie Lin
human	IHC	Cell Signal; Integration of exosomal miR-106a and mesothelial cells facilitates gastric cancer peritoneal dissemination.; Authors - Meng Zhu