Anticorps Polyclonal de lapin anti-TIMP1

TIMP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 26847-1-AP

Synonymes

TIMP-1, CLGI, Collagenase inhibitor, EPA, Erythroid-potentiating activity

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Galerie de données de validation

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  WB analysis using 26847-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26847-1-AP (TIMP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26847-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26847-1-AP (TIMP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 26847-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 26847-1-AP (TIMP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 26847-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 26847-1-AP (TIMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SKOV-3 using 26847-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using TIMP1 antibody (26847-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of HT-29 using 26847-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HT-29 cells using TIMP1 antibody (26847-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Applications testées

• Résultats positifs en WB: cellules A2780, cellules HT-29, cellules SKOV-3
• Résultats positifs en IHC: tissu de côlon de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SKOV-3, cellules HT-29

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

26847-1-AP cible TIMP1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TIMP1 Protéine recombinante Ag25395
• Nom complet: TIMP metallopeptidase inhibitor 1
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 23-28 kDa
• Numéro d’acquisition GenBank: BC000866
• Symbole du gène: TIMP1
• Identification du gène (NCBI): 7076
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TIMP1 is a member of the family of matrix metalloproteinase inhibitors, which contains four members (TIMP1, TIMP2, TIMP3, and TIMP4). Tissue inhibitors of metalloproteinases (TIMPs) are multifaceted molecules that exhibit properties beyond their classical proteinase inhibitory function. TIMP1 has several MMP-independent functions such as modulation of angiogenesis, promotion of cell proliferation, and inhibition of apoptosis. TIMP1 plays important role in cell cycle regulation and cancer progression. Recently, clinical studies have shown that the aberrant expression of TIMP1 is associated with an unfavorable prognosis in a series of tumors, such as gastric cancer, papillary thyroid carcinoma, cutaneous melanoma and breast cancer. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium.

Publications

Species	Application	Title
mouse	WB	BMC Cardiovasc Disord; Baricitinib represses the myocardial fibrosis via blocking JAK/STAT and TGF-β1 pathways in vivo and in vitro; Authors - Renlei Feng

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