Anticorps Polyclonal de lapin anti-TIMELESS

• Phare
• Validé par KD/KO

TIMELESS Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 14421-1-AP

Synonymes

hTIM, Protein timeless homolog, TIM, TIM1, TIMELESS, timeless homolog (Drosophila), TIMELESS1

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Galerie de données de validation

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  WB analysis using 14421-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14421-1-AP (TIMELESS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 14421-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14421-1-AP (TIMELESS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14421-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14421-1-AP (TIMELESS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 14421-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, cellules HeLa, cellules Jurkat, cellules NIH/3T3, cellules RAW 264.7
• Résultats positifs en IHC: tissu splénique humain, tissu cardiaque humain, tissu cérébral humain, tissu ovarien humain, tissu placentaire humain, tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 3 publications below

Informations sur le produit

14421-1-AP cible TIMELESS dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TIMELESS Protéine recombinante Ag5767
• Nom complet: timeless homolog (Drosophila)
• Masse moléculaire calculée: 139 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: BC050557
• Symbole du gène: TIMELESS
• Identification du gène (NCBI): 8914
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TIMELESS1 (TIM1) gene encodes protein timeless homolog belonging to timeless family. TIM1 is localized in nucleus and regulated by cell cycle. It expresses high levels in S, G2 and M phases, and low in G0 and G1 phases. TIM1 is involved in the circadian rhythm autoregulatory loop and is a crucial component of the circadian core oscillator, which includes the CRY proteins, CLOCK, NPAS2, etc. It is also involved in cell survival after DNA damage or replication stress and reported to play important role in epithelial cell morphogenesis and formation of branching tubules.

Publications

Species	Application	Title
human	WB,IHC	Cancer Lett; TIMELESS confers cisplatin resistance in nasopharyngeal carcinoma by activating the Wnt/β-catenin signaling pathway and promoting the epithelial mesenchymal transition.; Authors - Sai-Lan Liu ; KD Validated
human	WB	Cell Death Dis; MEX3A promotes the malignant progression of ovarian cancer by regulating intron retention in TIMELESS.; Authors - Fangfang Li ; KD Validated
human	WB	Cell Prolif; Circadian clock genes promote glioma progression by affecting tumour immune infiltration and tumour cell proliferation.; Authors - Zeyu Wang
human	WB,IHC	Gut Microbes; Exosomal-miR-129-2-3p derived from Fusobacterium nucleatum-infected intestinal epithelial cells promotes experimental colitis through regulating TIMELESS-mediated cellular senescence pathway; Authors - Shuchun Wei ; KD Validated
human	WB	Cell Signal; HMGB1-mediated transcriptional activation of circadian gene TIMELESS contributes to endometrial cancer progression through Wnt-β-catenin pathway; Authors - Zhaoxia Wang
human,mouse	WB,IHC	J Transl Med; TIMELESS promotes reprogramming of glucose metabolism in oral squamous cell carcinoma; Authors - Yafan Chen