Anticorps Monoclonal anti-TIA1

TIA1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

2C11F4

N° de cat : 68486-1-Ig

Synonymes

Nucleolysin TIA 1 isoform p40, p40 TIA 1, RNA binding protein TIA 1, TIA 1, TIA1



Applications testées

Résultats positifs en WBcellules HUVEC, cellules COLO 320, cellules HepG2, cellules HSC-T6, cellules hTERT-RPE1, cellules Jurkat, cellules K-562, cellules MOLT-4, cellules NIH/3T3

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

68486-1-Ig cible TIA1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène TIA1 Protéine recombinante Ag2778
Nom complet TIA1 cytotoxic granule-associated RNA binding protein
Masse moléculaire calculée 214 aa, 24 kDa, 43 kDa
Poids moléculaire observé38-40 kDa
Numéro d’acquisition GenBankBC015944
Symbole du gène TIA1
Identification du gène (NCBI) 7072
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TIA1, also named as p40-TIA-1, is involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). It possesses nucleolytic activity against cytotoxic lymphocyte target cells. TIA1 may be involved in apoptosis. Two isoforms of this protein exist - 41kDa and 42kDa. one of these was a missense variant (P362L) in TIA1. Similar to the ALS-related disease proteins TDP-43, hnRNPA1, and FUS, TIA1 is an RNA-binding protein containing a prionlike LCD and assembles into membrane-less organelles, including SGs. Postmortem neuropathology of five TIA1 mutations carriers showed a consistent pathological signature with numerous round, hyaline, TAR DNA-binding protein 43 (TDP-43)-positive inclusions.TIA1mutations significantly increased the propensity of TIA1 protein to undergo phase transition. In live cells,TIA1mutations delayed stress granule (SG) disassembly and promoted the accumulation of non-dynamic SGs that harbored TDP-43.

Protocole

Product Specific Protocols
WB protocol for TIA1 antibody 68486-1-IgDownload protocol
Standard Protocols
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