Anticorps Monoclonal anti-TIA1

TIA1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

2C11F4

N° de cat : 68486-1-Ig

Synonymes

Nucleolysin TIA 1 isoform p40, p40 TIA 1, RNA binding protein TIA 1, TIA 1, TIA1

---

Galerie de données de validation

• 

  WB analysis using 68486-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68486-1-Ig (TIA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules COLO 320, cellules HepG2, cellules HSC-T6, cellules hTERT-RPE1, cellules Jurkat, cellules K-562, cellules MOLT-4, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68486-1-Ig cible TIA1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TIA1 Protéine recombinante Ag2778
• Nom complet: TIA1 cytotoxic granule-associated RNA binding protein
• Masse moléculaire calculée: 214 aa, 24 kDa, 43 kDa
• Poids moléculaire observé: 38-40 kDa
• Numéro d’acquisition GenBank: BC015944
• Symbole du gène: TIA1
• Identification du gène (NCBI): 7072
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TIA1, also named as p40-TIA-1, is involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). It possesses nucleolytic activity against cytotoxic lymphocyte target cells. TIA1 may be involved in apoptosis. Two isoforms of this protein exist - 41kDa and 42kDa. one of these was a missense variant (P362L) in TIA1. Similar to the ALS-related disease proteins TDP-43, hnRNPA1, and FUS, TIA1 is an RNA-binding protein containing a prionlike LCD and assembles into membrane-less organelles, including SGs. Postmortem neuropathology of five TIA1 mutations carriers showed a consistent pathological signature with numerous round, hyaline, TAR DNA-binding protein 43 (TDP-43)-positive inclusions.TIA1mutations significantly increased the propensity of TIA1 protein to undergo phase transition. In live cells,TIA1mutations delayed stress granule (SG) disassembly and promoted the accumulation of non-dynamic SGs that harbored TDP-43.