Anticorps Polyclonal de lapin anti-THTPA

THTPA Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 15486-1-AP

Synonymes

thiamine triphosphatase, THTP, THTPA, THTPASE

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Galerie de données de validation

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  WB analysis of K-562 using 15486-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 15486-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15486-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15486-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15486-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 15486-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 15486-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 15486-1-AP (THTPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 15486-1-AP

  IP result of anti-THTPA (IP:15486-1-AP, 3ug; Detection:15486-1-AP 1:800) with K-562 cells lysate 1720ug.

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  IHC staining of human stomach cancer using 15486-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 15486-1-AP (THTPA antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach cancer using 15486-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 15486-1-AP (THTPA antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 15486-1-AP

  Immunofluorescent analysis of HepG2 cells, using THTPA antibody 15486-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules A431, cellules HeLa, cellules PC-3, tissu cérébral humain, tissu testiculaire de souris, tissu utérin de souris
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

15486-1-AP cible THTPA dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: THTPA Protéine recombinante Ag7834
• Nom complet: thiamine triphosphatase
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 26-35 kDa
• Numéro d’acquisition GenBank: BC002984
• Symbole du gène: THTPA
• Identification du gène (NCBI): 79178
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	PLoS One; Metabolic effects of acute thiamine depletion are reversed by rapamycin in breast and leukemia cells.; Authors - Shuqian Liu