Anticorps Polyclonal de lapin anti-THRSP

• Phare
• Validé par KD/KO

THRSP Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 13054-1-AP

Synonymes

S14, Spot 14 protein, SPOT14, THRSP

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Galerie de données de validation

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  WB analysis of mouse liver using 13054-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13054-1-AP (THRSP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 13054-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13054-1-AP (THRSP Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 13054-1-AP

  IP result of anti-THRSP (IP:13054-1-AP, 4ug; Detection:13054-1-AP 1:300) with mouse liver tissue lysate 6000ug.

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  IHC staining of human liver using 13054-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13054-1-AP (THRSP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 13054-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13054-1-AP (THRSP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13054-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13054-1-AP (THRSP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13054-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13054-1-AP (THRSP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13054-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13054-1-AP (THRSP antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 13054-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13054-1-AP (THRSP antibody) at dilution of 1:200 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris,
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:100-1:600
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

13054-1-AP cible THRSP dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: THRSP Protéine recombinante Ag3721
• Nom complet: thyroid hormone responsive (SPOT14 homolog, rat)
• Masse moléculaire calculée: 146 aa, 17 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC031989
• Symbole du gène: THRSP
• Identification du gène (NCBI): 7069
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

THRSP (Thyroid hormone-inducible hepatic protein)  is a protein that in humans is encoded by the THRSP gene. The protein encoded by this gene is similar to the gene product of S14, a rat gene whose expression is limited to liver and adipose tissue and is controlled by nutritional and hormonal factors. This gene has been shown to be expressed in liver and adipocytes, particularly in lipomatous modules. It is also found to be expressed in lipogenic breast cancers, which suggests a role in controlling tumor lipid metabolism (RefSeq). This antibody works well in IHC, WB and IF.

Publications

Species	Application	Title
human	WB	Mol Med; Insulin-inducible THRSP maintains mitochondrial function and regulates sphingolipid metabolism in human adipocytes.; Authors - Maria A Ahonen
human	WB	Sci Rep; Elevated serum S14 levels are associated with more severe liver steatosis by ultrasonography.; Authors - Wen-Ti Lin
mouse	WB	Mol Cell Endocrinol; MiR-451a attenuates free fatty acids-mediated hepatocyte steatosis by targeting the thyroid hormone responsive spot 14 gene.; Authors - Ni Zeng ; KD Validated
		Medicine (Baltimore); Serum Spot 14 concentration is negatively associated with thyroid-stimulating hormone level.; Authors - Yen-Ting Chen
		PLoS One; The serum level of a novel lipogenic protein Spot 14 was reduced in metabolic syndrome.; Authors - Yen-Ting Chen