Anticorps Polyclonal de lapin anti-TGM3

TGM3 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 21406-1-AP

Synonymes

TG(E), TGase 3, TGase E, TGE, TGM3, Transglutaminase 3, Transglutaminase E

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Galerie de données de validation

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  WB analysis using 21406-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21406-1-AP (TGM3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules SMMC-7721,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

21406-1-AP cible TGM3 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TGM3 Protéine recombinante Ag16131
• Nom complet: transglutaminase 3 (E polypeptide, protein-glutamine-gamma-glutamyltransferase)
• Masse moléculaire calculée: 693 aa, 77 kDa
• Poids moléculaire observé: 77 kDa; 30-35 kDa; 44-50 kDa
• Numéro d’acquisition GenBank: BC109076
• Symbole du gène: TGM3
• Identification du gène (NCBI): 7053
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TGM3 (Transglutaminase 3) is a Protein Coding gene. Diseases associated with TGM3 include Uncombable Hair Syndrome 2 and Uncombable Hair Syndrome 1. Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene consists of two polypeptide chains activated from a single precursor protein by proteolysis. The encoded protein is involved the later stages of cell envelope formation in the epidermis and hair follicle. TG3 is expressed as an inactive 77 kDa zymogen which must be activated by limited proteolytic processing into two fragments (44 kDa and 30 kDa) of which the larger, N-terminal fragment carries the catalytic activity (PMID: 35328331).