Anticorps Polyclonal de lapin anti-TGFBR2

TGFBR2 Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 27212-1-AP

Synonymes

AAT3, FAA3, LDS1B, LDS2B, MFS2, RIIC, TAAD2, TbetaR II, TGF beta receptor type 2, TGF beta receptor type II, TGF beta type II receptor, TGFbeta RII, TGFBR2, TGFR 2

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Galerie de données de validation

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  WB analysis using 27212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27212-1-AP (TGFBR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 27212-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27212-1-AP (TGFBR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 27212-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27212-1-AP (TGFBR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of THP-1 using 27212-1-AP

  1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human TGFBR2 (27212-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules Colo320, cellules MCF-7
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules THP-1,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

27212-1-AP cible TGFBR2 dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TGFBR2 Protéine recombinante Ag25767
• Nom complet: transforming growth factor, beta receptor II (70/80kDa)
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 65-80 kDa
• Numéro d’acquisition GenBank: BC040499
• Symbole du gène: TGFBR2
• Identification du gène (NCBI): 7048
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TGF beta Receptor II (TGFBR2) is a member of the transforming growth factor-β (TGF-β) superfamily, which are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (PMID: 10974075). TGFBR2 result in both SMAD4-dependent constraint of proliferation and SMAD4-independent activation of apoptosis (PMID: 29396446). It has some isoforms with the molecular weight of 70-80 kDa and ~90 kDa after glycosylated.

Publications

Species	Application	Title
mouse	FC	Cell Commun Signal; The activation of PPARγ enhances Treg responses through up-regulating CD36/CPT1-mediated fatty acid oxidation and subsequent N-glycan branching of TβRII/IL-2Rα.; Authors - Yumeng Miao
human	WB	Reprod Sci; Maternal Perinatal Exposure to Dibutyl Phthalate Promotes Ovarian Dysfunction in Adult Female Offspring via Downregulation of TGF-β2 and TGF-β3.; Authors - Jing Zhang
mouse	WB	Acta Histochem; Inhibition of glutamine transporter ASCT2 mitigates bleomycin-induced pulmonary fibrosis in mice; Authors - Naqi Lian
human,mouse	WB,IHC	Front Pharmacol; 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside ameliorates bleomycin-induced pulmonary fibrosis via regulating pro-fibrotic signaling pathways; Authors - Tsung-Teng Huang
sheep	WB	Theriogenology; Exploration the role of INHBA in Hu sheep granulosa cells using RNA-Seq; Authors - Yongjin Bao
human	WB	Cell Chem Biol; Therapeutic potency of compound RMY-205 for pulmonary fibrosis induced by SARS-CoV-2 nucleocapsid protein; Authors - Zhi-Yuan Zhang